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URL: http://www.addgene.org/251503
Proper Citation: RRID:Addgene_251503
Insert Name: SAGE cassette
Bacterial Resistance: Ampicillin and Gentamicin
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin
Comments: A C to T mutation at nt 33 of SAGE cassette was found, this does not impact the intended use of the plasmid. Alternative name: pRC414.v2 Usage/Comments: This plasmid is used to generate a cSAGE-compatible base strain by integrating the poly-attB landing pad cassette into the host genome using a Tn7-based strategy. The landing pad contains multiple orthogonal serine recombinase attachment sites (attB), enabling subsequent site-specific integration of genetic payloads via cSAGE. Once integrated, the host strain becomes compatible with Bxb1, R4, TG1, and other serine recombinases for modular genome engineering. This plasmid is used only for installation of the landing pad and is not required for downstream integration steps after the base strain is constructed. Please visit https://doi.org/10.64898/2026.04.17.717921 for bioRxiv preprint.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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