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URL: http://www.addgene.org/131318
Proper Citation: RRID:Addgene_131318
Insert Name: Full-length tetO array (XbaI-NheI fragment of pLAU44)
Organism: Synthetic
Bacterial Resistance: Ampicillin and Gentamicin
Defining Citation: PMID:31821884
Vector Backbone Description: Backbone Size:6803; Vector Backbone:pMF272; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Gentamicin
Comments: TetO arrays are unstable and dual selection with Ampicillin and Gentamycin are essential for the maintenance of the array. Confirm full-length plasmid by restriction digest and Sanger sequencing of any clones prior to use. Some minor sequence discrepancies located in the TetO repeat array exist between Sanger and NGS based data. These are not of functional concern for use of the plasmid.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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