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In this report we describe a human pluripotent stem cell-derived vascular progenitor (MesoT) cell of the mesothelium lineage. MesoT cells are multipotent and generate smooth muscle cells, endothelial cells, and pericytes and self-assemble into vessel-like networks in vitro. MesoT cells transplanted into mechanically damaged neonatal mouse heart migrate into the injured tissue and contribute to nascent coronary vessels in the repair zone. When seeded onto decellularized vascular scaffolds, MesoT cells differentiate into the major vascular lineages and self-assemble into vasculature capable of supporting peripheral blood flow following transplantation. These findings demonstrate in vivo functionality and the potential utility of MesoT cells in vascular engineering applications.
Pubmed ID: 30840882 RIS Download
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Set of software modules for performing common ChIP-seq data analysis tasks across the whole genome, including positional correlation analysis, peak detection, and genome partitioning into signal-rich and signal-poor regions. The tools are designed to be simple, fast and highly modular. Each program carries out a well defined data processing procedure that can potentially fit into a pipeline framework. ChIP-Seq is also freely available on a Web interface.
View all literature mentionsSoftware environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.
View all literature mentionsOriginal SAMTOOLS package has been split into three separate repositories including Samtools, BCFtools and HTSlib. Samtools for manipulating next generation sequencing data used for reading, writing, editing, indexing,viewing nucleotide alignments in SAM,BAM,CRAM format. BCFtools used for reading, writing BCF2,VCF, gVCF files and calling, filtering, summarising SNP and short indel sequence variants. HTSlib used for reading, writing high throughput sequencing data.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsSoftware ultrafast memory efficient tool for aligning sequencing reads. Bowtie is short read aligner.
View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsA commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.
View all literature mentionsSoftware package for high-performance read alignment, quantification and mutation discovery.General purpose read aligner which can be used to map both genomic DNA-seq reads and RNA-seq reads. Subread aligner as fast, accurate and scalable read mapping by seed-and-vote.These programs were also implemented in Bioconductor R package Rsubread.
View all literature mentionsSoftware package for the analysis of gene expression microarray data, especially the use of linear models for analyzing designed experiments and the assessment of differential expression.
View all literature mentionsA generic alignment format for storing read alignments against reference sequences, supporting short and long reads (up to 128 Mbp) produced by different sequencing platforms.
View all literature mentionsBioconductor software package for Empirical analysis of Digital Gene Expression data in R. Used for differential expression analysis of RNA-seq and digital gene expression data with biological replication.
View all literature mentionsQuality control software that perform checks on raw sequence data coming from high throughput sequencing pipelines. This software also provides a modular set of analyses which can give a quick impression of the quality of the data prior to further analysis.
View all literature mentionsSystem that classifies genes by their functions, using published scientific experimental evidence and evolutionary relationships to predict function even in absence of direct experimental evidence. Orthologs view is curated orthology relationships between genes for human, mouse, rat, fish, worm, and fly.
View all literature mentionsSoftware application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.
View all literature mentionsSoftware package for interactive graphics for gene expression analysis. Generates interactive visualisations for analysis of RNA-sequencing data.
View all literature mentionsSystem that classifies genes by their functions, using published scientific experimental evidence and evolutionary relationships to predict function even in absence of direct experimental evidence. Orthologs view is curated orthology relationships between genes for human, mouse, rat, fish, worm, and fly.
View all literature mentionsThis isotype control targets Not Applicable
View all literature mentionsThis monoclonal targets Human Wilms Tumor Protein
View all literature mentionsThis polyclonal targets H3K4me1
View all literature mentionsThis isotype control targets Not Applicable
View all literature mentionsThis isotype control targets not applicable
View all literature mentionsThis unknown targets VEGF Receptor 2
View all literature mentionsThis polyclonal targets H3K27ac
View all literature mentionsThis monoclonal targets Mouse IgG1 k
View all literature mentionsThis polyclonal targets SOX2
View all literature mentionsThis polyclonal targets Human TGN46
View all literature mentionsThis unknown targets Mouse IgG1 Allophycocyanin Isotype Control (Clone 11711)
View all literature mentionsThis monoclonal targets CD44
View all literature mentionsThis monoclonal targets Human NG2 / MCSP
View all literature mentionsThis monoclonal targets CD105 (Endoglin)
View all literature mentionsThis monoclonal targets Human NG2
View all literature mentionsThis monoclonal targets CD31
View all literature mentionsThis monoclonal targets CD31
View all literature mentionsThis polyclonal targets CD31
View all literature mentionsThis polyclonal targets Von Willebrand Factor
View all literature mentionsThis monoclonal targets MYH11
View all literature mentionsThis polyclonal targets alpha smooth muscle Actin
View all literature mentionsThis monoclonal targets Actin, alpha, Smooth Muscle
View all literature mentionsThis polyclonal targets E-Cadherin
View all literature mentionsThis monoclonal targets ZO-1
View all literature mentionsThis monoclonal targets Human TBX18 MAb (Clone 635305)
View all literature mentionsThis monoclonal targets Human NKX2.5
View all literature mentionsThis monoclonal targets Fast Myosin Skeletal Heavy chain
View all literature mentionsThis unknown targets VE-Cadherin
View all literature mentionsThis polyclonal targets Human Islet-1
View all literature mentionsThis monoclonal targets CD105
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Goat IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Goat IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Vimentin antibody [EPR3776]
View all literature mentionsThis polyclonal targets Human VE Cadherin
View all literature mentionsThis monoclonal targets Calponin antibody produced in mouse
View all literature mentionsCell line I 3 is a Embryonic stem cell with a species of origin Homo sapiens
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name STOCK Wt1tm2(cre/ERT2)Wtp/J from IMSR.
View all literature mentionsCell line WA01 is a Embryonic stem cell with a species of origin Homo sapiens
View all literature mentionsCell line WA07 is a Embryonic stem cell with a species of origin Homo sapiens
View all literature mentionsCell line WA09 is a Embryonic stem cell with a species of origin Homo sapiens (Human)
View all literature mentionsThis unknown targets VEGF Receptor 2
View all literature mentionsThis polyclonal targets H3K4me1
View all literature mentionsThis polyclonal targets H3K27ac
View all literature mentionsCell line I 3 is a Embryonic stem cell with a species of origin Homo sapiens
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name STOCK Wt1tm2(cre/ERT2)Wtp/J from IMSR.
View all literature mentionsThis isotype control targets Not Applicable
View all literature mentionsThis isotype control targets not applicable
View all literature mentionsThis isotype control targets Not Applicable
View all literature mentionsThis monoclonal targets Mouse IgG1 k
View all literature mentionsThis monoclonal targets Human Wilms Tumor Protein
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