Cervical cancer is a major public health problem and research using cell culture models has improved understanding of this disease. The human cervix contains three anatomic regions; ectocervix with stratified squamous epithelium, endocervix with secretory epithelium, and transformation zone (TZ) with metaplastic cells. Most cervical cancers originate within the TZ. However, little is known about the biology of TZ cells or why they are highly susceptible to carcinogenesis. The goal of this study was to develop and optimize methods to compare growth and differentiation of cells cultured from ectocervix, TZ or endocervix. We examined the effects of different serum-free media on cell attachment, cell growth and differentiation, and cell population doublings in monolayer culture. We also optimized conditions for organotypic culture of cervical epithelial cells using collagen rafts with human cervical stromal cells. Finally, we present a step-by-step protocol for culturing cells from each region of human cervix.
Pubmed ID: 30609028 RIS Download
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This monoclonal targets
View all literature mentionsThis monoclonal targets IgG
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis monoclonal targets Human Cytokeratin 14
View all literature mentionsThis monoclonal targets MUC5AC
View all literature mentionsThis monoclonal targets Cytokeratin 18
View all literature mentionsThis polyclonal targets IgG H&L
View all literature mentionsCell line 3T3-J2 is a Spontaneously immortalized cell line with a species of origin Mus musculus (Mouse)
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