In vivo, the primary molecular mechanotransductive events mechanically initiating cell differentiation remain unknown. Here we find the molecular stretching of the highly conserved Y654-β-catenin-D665-E-cadherin binding site as mechanically induced by tissue strain. It triggers the increase of accessibility of the Y654 site, target of the Src42A kinase phosphorylation leading to irreversible unbinding. Molecular dynamics simulations of the β-catenin/E-cadherin complex under a force mimicking a 6 pN physiological mechanical strain predict a local 45% stretching between the two α-helices linked by the site and a 15% increase in accessibility of the phosphorylation site. Both are quantitatively observed using FRET lifetime imaging and non-phospho Y654 specific antibody labelling, in response to the mechanical strains developed by endogenous and magnetically mimicked early mesoderm invagination of gastrulating Drosophila embryos. This is followed by the predicted release of 16% of β-catenin from junctions, observed in FRAP, which initiates the mechanical activation of the β-catenin pathway process.
Pubmed ID: 30024850 RIS Download
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THIS RESOURCE IS NO LONGER IN SERVICE, documented on February 1st, 2022. Software application for genetic analysis of classical biometric traits like blood pressure or height that are caused by a combination of polygenic inheritance and complex environmental forces. (entry from Genetic Analysis Software)
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsA commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.
View all literature mentionsAn antibody supplier which banks and distributes hybridomas and monoclonal antibodies for use in research. The bank includes antibodies against targets such as GFP, transcription factors, stem cells, and human.
View all literature mentionsParallel molecular dynamics code designed for high-performance simulation of large biomolecular systems. NAMD uses the popular molecular graphics program VMD for simulation setup and trajectory analysis, but is also file-compatible with AMBER, CHARMM, and X-PLOR.
View all literature mentionsThis monoclonal targets Mouse Drosophila Armadillo Protein
View all literature mentionsThis monoclonal targets cadherin, DE- (extracellular domain)
View all literature mentionsThis polyclonal targets CTNNB1 (Ab-654) antibody produced in rabbit
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD8338}v17643 from Flybase.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=matalpha4-GAL-VP16}V37 from BDSC.
View all literature mentionsDrosophila melanogaster with name Oregon-R-C from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=arm-GFP.P}57 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=matalpha4-GAL-VP16}V37 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD8338}v17643 from Flybase.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=arm-GFP.P}57 from BDSC.
View all literature mentionsThis monoclonal targets cadherin, DE- (extracellular domain)
View all literature mentionsThis monoclonal targets Mouse Drosophila Armadillo Protein
View all literature mentionsThis polyclonal targets CTNNB1 (Ab-654) antibody produced in rabbit
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=arm-GFP.P}57 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=matalpha4-GAL-VP16}V37 from BDSC.
View all literature mentionsThis polyclonal targets CTNNB1 (Ab-654) antibody produced in rabbit
View all literature mentionsThis monoclonal targets cadherin, DE- (extracellular domain)
View all literature mentionsThis monoclonal targets Mouse Drosophila Armadillo Protein
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD8338}v17643 from Flybase.
View all literature mentionsDrosophila melanogaster with name Oregon-R-C from BDSC.
View all literature mentions