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Top3β is an RNA topoisomerase that works with fragile X syndrome protein to promote synapse formation.

Nature neuroscience | Sep 28, 2013

Topoisomerases are crucial for solving DNA topological problems, but they have not been linked to RNA metabolism. Here we show that human topoisomerase 3β (Top3β) is an RNA topoisomerase that biochemically and genetically interacts with FMRP, a protein that is deficient in fragile X syndrome and is known to regulate the translation of mRNAs that are important for neuronal function, abnormalities of which are linked to autism. Notably, the FMRP-Top3β interaction is abolished by a disease-associated mutation of FMRP, suggesting that Top3β may contribute to the pathogenesis of mental disorders. Top3β binds multiple mRNAs encoded by genes with neuronal functions linked to schizophrenia and autism. Expression of one such gene, that encoding protein tyrosine kinase 2 (ptk2, also known as focal adhesion kinase or FAK), is reduced in the neuromuscular junctions of Top3β mutant flies. Synapse formation is defective in Top3β mutant flies and mice, as well as in FMRP mutant flies and mice. Our findings suggest that Top3β acts as an RNA topoisomerase and works with FMRP to promote the expression of mRNAs that are crucial for neurodevelopment and mental health.

Pubmed ID: 23912945 RIS Download

Mesh terms: Animals | Animals, Genetically Modified | Cells, Cultured | Chickens | DNA Topoisomerases, Type I | Drosophila | Drosophila Proteins | Embryo, Mammalian | Eye | Fragile X Mental Retardation Protein | Gene Expression Regulation | Humans | Mice | Mice, Inbred C57BL | Nerve Tissue Proteins | Neurogenesis | Neuromuscular Junction | Neurons | RNA-Binding Proteins | Transfection

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Associated grants

  • Agency: NINDS NIH HHS, Id: P30 NS050274
  • Agency: NIA NIH HHS, Id: Z01 AG000657
  • Agency: NIA NIH HHS, Id: Z01 AG000657-08
  • Agency: NINDS NIH HHS, Id: NS050274
  • Agency: NINDS NIH HHS, Id: R01 NS082563
  • Agency: Canadian Institutes of Health Research, Id: MOP-79368
  • Agency: Intramural NIH HHS, Id: ZIA AG000365-08
  • Agency: Intramural NIH HHS, Id: Z01 AG000657-08
  • Agency: NIA NIH HHS, Id: K24 AG042489

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