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Dapper antagonist of catenin-1 cooperates with Dishevelled-1 during postsynaptic development in mouse forebrain GABAergic interneurons.

PloS one | Jul 4, 2013

Synaptogenesis has been extensively studied along with dendritic spine development in glutamatergic pyramidal neurons, however synapse development in cortical interneurons, which are largely aspiny, is comparatively less well understood. Dact1, one of 3 paralogous Dact (Dapper/Frodo) family members in mammals, is a scaffold protein implicated in both the Wnt/β-catenin and the Wnt/Planar Cell Polarity pathways. We show here that Dact1 is expressed in immature cortical interneurons. Although Dact1 is first expressed in interneuron precursors during proliferative and migratory stages, constitutive Dact1 mutant mice have no major defects in numbers or migration of these neurons. However, cultured cortical interneurons derived from these mice have reduced numbers of excitatory synapses on their dendrites. We selectively eliminated Dact1 from mouse cortical interneurons using a conditional knock-out strategy with a Dlx-I12b enhancer-Cre allele, and thereby demonstrate a cell-autonomous role for Dact1 during postsynaptic development. Confirming this cell-autonomous role, we show that synapse numbers in Dact1 deficient cortical interneurons are rescued by virally-mediated re-expression of Dact1 specifically targeted to these cells. Synapse numbers in these neurons are also rescued by similarly targeted expression of the Dact1 binding partner Dishevelled-1, and partially rescued by expression of Disrupted in Schizophrenia-1, a synaptic protein genetically implicated in susceptibility to several major mental illnesses. In sum, our results support a novel cell-autonomous postsynaptic role for Dact1, in cooperation with Dishevelled-1 and possibly Disrupted in Schizophrenia-1, in the formation of synapses on cortical interneuron dendrites.

Pubmed ID: 23826333 RIS Download

Mesh terms: Adaptor Proteins, Signal Transducing | Animals | Cell Movement | Dendrites | Dishevelled Proteins | GABAergic Neurons | Gene Expression Regulation, Developmental | Interneurons | Intracellular Signaling Peptides and Proteins | Lentivirus | Mice | Mice, Knockout | Mice, Mutant Strains | Nerve Tissue Proteins | Neural Inhibition | Phosphoproteins | Prosencephalon | Protein Binding | Synapses

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Associated grants

  • Agency: NIDDK NIH HHS, Id: P30 DK063720
  • Agency: NIMH NIH HHS, Id: T32MH089920
  • Agency: NIMH NIH HHS, Id: R21 MH085995
  • Agency: NIMH NIH HHS, Id: T32 MH089920
  • Agency: NIMH NIH HHS, Id: R21MH085995
  • Agency: NIMH NIH HHS, Id: R01MH081880
  • Agency: NIMH NIH HHS, Id: R37MH049428
  • Agency: NIGMS NIH HHS, Id: R25 GM056847
  • Agency: NIMH NIH HHS, Id: R37 MH049428
  • Agency: NIGMS NIH HHS, Id: R25GM56847

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