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Filamin B represses chondrocyte hypertrophy in a Runx2/Smad3-dependent manner.

FILAMIN B, which encodes a cytoplasmic actin binding protein, is mutated in several skeletal dysplasias. To further investigate how an actin binding protein influences skeletogenesis, we generated mice lacking intact Filamin B. As observed in spondylocarpotarsal synostosis syndrome patients, Filamin B mutant mice display ectopic mineralization in many cartilaginous elements. This aberrant mineralization is due to ectopic chondrocyte hypertrophy similar to that seen in mice expressing Runx2 in chondrocytes. Accordingly, removing one copy of Runx2 rescues the Filamin B mutant phenotype, indicating that Filamin B is a regulator of Runx2 function during chondrocyte differentiation. Filamin B binds Smad3, which is known to interact with Runx2. Smad3 phosphorylation is increased in the mutant mice. Thus, Filamin B inhibits Runx2 activity, at least in part, through the Smad3 pathway. Our results uncover the involvement of actin binding proteins during chondrogenesis and provide a molecular basis to a human genetic disease.

Pubmed ID: 17606870

Authors

  • Zheng L
  • Baek HJ
  • Karsenty G
  • Justice MJ

Journal

The Journal of cell biology

Publication Data

July 2, 2007

Associated Grants

  • Agency: NIEHS NIH HHS, Id: P01 ES11253
  • Agency: NIAMS NIH HHS, Id: R01 AR 045548
  • Agency: NICHD NIH HHS, Id: U01 HD39372

Mesh Terms

  • Animals
  • Chondrocytes
  • Contractile Proteins
  • Core Binding Factor Alpha 1 Subunit
  • Filamins
  • Gene Expression Regulation, Developmental
  • Glutathione Transferase
  • Hypertrophy
  • In Situ Hybridization
  • Mice
  • Mice, Inbred C57BL
  • Microfilament Proteins
  • Models, Biological
  • Mutation
  • Recombinant Proteins
  • Smad3 Protein