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The generation of a conditional Fmr1 knock out mouse model to study Fmrp function in vivo.

Neurobiology of disease | Mar 28, 2006

http://www.ncbi.nlm.nih.gov/pubmed/16257225

The FMR1 gene, mutated in Fragile X syndrome patients, has been modeled in mice with a neomycin cassette inserted in exon 5 of the mouse Fmr1 gene creating an Fmr1 knockout (Fmr1 KO) allele. This results in animals lacking Fmr1 protein (Fmrp) expression in all tissues. We have created a new, more versatile Fmr1 in vivo KO model (Fmr1 KO2) and generated conditional Fmr1 KO (CKO) mice by flanking the promoter and first exon of Fmr1 with lox P sites. This enables us to create a null allele in specific cell types and at specific time points by crossing Fmr1 CKO mice with tissue specific or inducible cre-recombinase expressing mice. The new Fmr1 KO2 line does not express any Fmrp and also lacks detectable Fmr1 transcripts. Crossing the Fmr1 CKO line with a Purkinje cell-specific cre-recombinase expresser produces mice that are null for Fmr1 in Purkinje neurons but wild type in all other cell types.

Pubmed ID: 16257225 RIS Download

Mesh terms: Animals | Blotting, Western | Disease Models, Animal | Fragile X Mental Retardation Protein | Fragile X Syndrome | Immunohistochemistry | Mice | Mice, Knockout | Purkinje Cells | Reverse Transcriptase Polymerase Chain Reaction

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