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The B-box dominates SAP-1-SRF interactions in the structure of the ternary complex.

The EMBO journal | Jun 15, 2001

http://www.ncbi.nlm.nih.gov/pubmed/11406578

The serum response element (SRE) is found in several immediate-early gene promoters. This DNA sequence is necessary and sufficient for rapid transcriptional induction of the human c-fos proto-oncogene in response to stimuli external to the cell. Full activation of the SRE requires the cooperative binding of a ternary complex factor (TCF) and serum response factor (SRF) to their specific DNA sites. The X-ray structure of the human SAP-1-SRF-SRE DNA ternary complex was determined (Protein Data Bank code 1hbx). It shows SAP-1 TCF bound to SRF through interactions between the SAP-1 B-box and SRF MADS domain in addition to contacts between their respective DNA-binding motifs. The SAP-1 B-box is part of a flexible linker of which 21 amino acids become ordered upon ternary complex formation. Comparison with a similar region from the yeast MATalpha2-MCM1-DNA complex suggests a common binding motif through which MADS-box proteins may interact with additional factors such as Fli-1.

Pubmed ID: 11406578 RIS Download

Mesh terms: Amino Acid Sequence | DNA | DNA-Binding Proteins | Glycoproteins | Humans | Models, Molecular | Molecular Sequence Data | Nuclear Proteins | Nucleic Acid Conformation | Protein Structure, Secondary | Regulatory Sequences, Nucleic Acid | Saposins | Serum Response Factor | Sphingolipid Activator Proteins