URL: http://www.addgene.org/15205
Proper Citation: RRID:Addgene_15205
Insert Name: Chx10 3kb AP reporter
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15893976
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The 3Kb-AP reporter was constructed using a genomic BamHI–SacII fragment isolated from a Chx10 BAC (Rowan and Cepko, 2004) and subcloned into pBluescript (Stratagene). An SV40 intervening sequence (IVS) was isolated from pNASSbeta (Clontech) digested with EcoRI and blunt cloned into the 3' SacII restriction site. The full length alkaline phosphatase (AP) reporter construct was isolated from the SV40all plasmid (gift of S. Dymecki).
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Source: Addgene