URL: http://www.addgene.org/10879
Proper Citation: RRID:Addgene_10879
Insert Name: non-hairpin 18bp
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16564017
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: This plasmid is recommended by The RNAi Consortium for use as a non-hairpin control http://www.broad.mit.edu/genome_bio/trc/ The 18bp between AgeI and EcoRI of this plasmid are CCGCAGGTATGCACGCGT. The link to the author's map shows the key features of this vector before the 18bp were cloned in. Also, see Addgene's pLKO.1 protocol http://www.addgene.org/plko on how to use the pLKO.1 vector.
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Source: Addgene