URL: http://antibodyregistry.org/AB_625998
Proper Citation: (GeneTex Cat# GTX80746, RRID:AB_625998)
Target Antigen: NTS1 antibody
Host Organism: guinea pig
Clonality: polyclonal
Comments: Discontinued; manufacturer recommendations: Immunocytochemistry. Immunocytochemistry 1:1000 (Dilutions listed only as a recommendation. Optimal dilution should be determined by investigator.) Immunocytochemistry Transfected COS-7 cells, plated on poly-L-lysine-coated glass coverslips, were fixed for 20 minutes with 4 paraformaldehyde in 0.1 M phosphate buffer (PB), pH 7.4, rinsed with 0.1 M Trisma base-buffered saline (TBS), pH 7.4, and preincubated for 30 minutes at RT with a blocking solution consisting of 5 normal goat serum (NGS), 2 BSA, and 0.1 Triton X-100 in 0.1 M TBS. Cells were then incubated overnight at 4°C with the anti-NTS1 peptide antiserum diluted 1: 1,000 in 0.1 M TBS, pH 7.4, containing 1 NGS and 0.05 Triton X-100. After washing with TBS, cells were incubated for 1 hour at RT with appropriate seconda
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No rating or validation information has been found for NTS1 antibody.
Discontinued
Discontinued at GeneTex
Source: Antibody Registry
