URL: http://antibodyregistry.org/AB_2616562
Proper Citation: (Risold PY Cat# 1153, RRID:AB_2616562)
Target Antigen: Synthetic Salmon MCH; full 17 amino acid sequence: DTMRKMVGRVYRPCWEV
Host Organism: rabbit
Clonality: polyclonal
Comments: Antibody prepared in 1987 by Risold and Fellmann, against the synthetic peptide coupled to the bovine serum albumin.Specificity of the antibody has been verified using liquid phase inhibition, dot blot, affinity column. We also verified the co-expression in the same lateral hypothalamic neurons of the peptide by immunohistochemistry and its ARNm by in situ hybridization in several species including rat. Fellmann D, Bugnon C, Risold PY. 1987. Unrelated peptide immunoreactivities coexist in neurons of the rat lateral dorsal hypothalamus: human growth hormone-releasing factor1-37-, salmon melanin-concentrating hormone- and alpha-melanotropin-like substances. Neurosci Lett 74:275–280. Risold PY, Fellmann D, Rivier J, Vale W, Bugnon C. 1992. Immunoreactivities for antisera to three putative neuropeptides of the rat melanin-concentrating hormone precursor are coexpressed in neurons of the rat lateral dorsal hypothalamus. Neurosci Lett 136:145–149.
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