URL: http://www.addgene.org/60954
Proper Citation: RRID:Addgene_60954
Insert Name: KRAB-dCas9-P2A-mCherry fusion
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25307932
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:9000; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: Please note that this plasmid contains a different promoter compared to the inducible KRAB-dCas9-P2A-mCherry construct described in Figure 5A of the associated publication (Gilbert et al., 2014). To create an inducible version of KRAB-dCas9-P2A-mCherry, subclone the insert into a backbone with an inducible promoter, such as pTRE3G. For stable expression of KRAB-dCas9 as described in Gilbert et al., 2014, please see pHR-SFFV-dCas9-BFP-KRAB (Addgene plasmid #46911).
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Source: Addgene