URL: http://www.addgene.org/45598
Proper Citation: RRID:Addgene_45598
Insert Name: c-Myc T58A
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15048125
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Alternate plasmid names: pD40-His-c-MycT58A; pcDNA-DEST40 Myc T58A Plasmid construction information: Point mutations were introduced into the murine c-Myc sequence in the CMV–Myc plasmid (Hann et al., Genes Dev. 1994, PMID: 7958908) using the GeneEditor kit (Promega) per manufacturer's instructions using oligonucletide MycT58A, 5′-CTGCTTCCCGCCCCGCCCCTGTC-3′. The His6-tagged c-MycT58A plasmid was then created by PCR amplification with a CACC 5′ extension added to the 5′ primer for cloning into the TOPO entry clone from Invitrogen (Carlsbad, CA). This sequence was then moved into the pDEST-40 mammalian expression vector using Gateway technology (Invitrogen), creating pD40-His-c-MycT58A. For more plasmid information (map and sequence), see the wildtype version, pD40-His/V5-c-Myc (Addgene plasmid 45597) For additional usage information, see the associated article (Yeh et al. Nat Cell Biol. 2004) as well as Arnold et al. EMBO 2009 (PMID: 19131971).
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Source: Addgene