URL: http://www.addgene.org/39196
Proper Citation: RRID:Addgene_39196
Insert Name: Firefly Luciferase Luc2P
Bacterial Resistance: Ampicillin
Defining Citation: PMID:
Vector Backbone Description: Vector Backbone:pHIV-Zsgreen; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The backbone plasmid, HIV-Zsgreen, was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Zsgreen. Luciferase (Luc2P) was cloned into the EcoRI and XbaI sites to generate this bicistronic lentivirus that expresses both Luciferase and Zsgreen.
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Source: Addgene