URL: http://www.addgene.org/123366
Proper Citation: RRID:Addgene_123366
Insert Name: U6:3-gRNA
Organism: Drosophila melanogaster
Bacterial Resistance: Ampicillin
Defining Citation: PMID:
Vector Backbone Description: Backbone Marker:Fillip Port; Vector Backbone:pCFD3-dU6-3gRNA (Addgene 49410); Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: When describing the use of this plasmid in publications, please state that it is derived from pCFD3 and cite: Port et al. Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):E2967-76. doi: 10.1073/pnas.1405500111 In pCFD3.1, the BbsI sites have been mutated in mini-white, which allows use of the remaining BbsI sites for gRNA cloning (using the protocol for pCFD3 (https://www.crisprflydesign.org/plasmids/)).
Expand AllWe found {{ ctrl2.mentions.total_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
Find mentions based on location
{{ ctrl2.mentions.errors.location }}
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for pCFD3.1-w-dU6:3gRNA.
No alerts have been found for pCFD3.1-w-dU6:3gRNA.
Source: Addgene