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In Drosophila, 23-30 nt long PIWI-interacting RNAs (piRNAs) direct the protein Piwi to silence germline transposon transcription. Most germline piRNAs derive from dual-strand piRNA clusters, heterochromatic transposon graveyards that are transcribed from both genomic strands. These piRNA sources are marked by the heterochromatin protein 1 homolog Rhino (Rhi), which facilitates their promoter-independent transcription, suppresses splicing, and inhibits transcriptional termination. Here, we report that the protein Maelstrom (Mael) represses canonical, promoter-dependent transcription in dual-strand clusters, allowing Rhi to initiate piRNA precursor transcription. Mael also represses promoter-dependent transcription at sites outside clusters. At some loci, Mael repression requires the piRNA pathway, while at others, piRNAs play no role. We propose that by repressing canonical transcription of individual transposon mRNAs, Mael helps Rhi drive non-canonical transcription of piRNA precursors without generating mRNAs encoding transposon proteins.
Pubmed ID: 30527661 RIS Download
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Database of biological data related to a single initiative, originating from a single organization or from a consortium. A BioProject record provides users a single place to find links to the diverse data types generated for that project. It is a searchable collection of complete and incomplete (in-progress) large-scale sequencing, assembly, annotation, and mapping projects for cellular organisms. Submissions are supported by a web-based Submission Portal. The database facilitates organization and classification of project data submitted to NCBI, EBI and DDBJ databases that captures descriptive information about research projects that result in high volume submissions to archival databases, ties together related data across multiple archives and serves as a central portal by which to inform users of data availability. BioProject records link to corresponding data stored in archival repositories. The BioProject resource is a redesigned, expanded, replacement of the NCBI Genome Project resource. The redesign adds tracking of several data elements including more precise information about a project''''s scope, material, and objectives. Genome Project identifiers are retained in the BioProject as the ID value for a record, and an Accession number has been added. Database content is exchanged with other members of the International Nucleotide Sequence Database Collaboration (INSDC). BioProject is accessible via FTP.
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View all literature mentionsA generic alignment format for storing read alignments against reference sequences, supporting short and long reads (up to 128 Mbp) produced by different sequencing platforms.
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View all literature mentionsDrosophila melanogaster with name w[*]; armi[72.1]/TM6C, Sb[1] Tb[1] from BDSC.
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View all literature mentionsDrosophila melanogaster with name w[1118]; P{ry[+t7.2]=ry11}piwi[2]/CyO, P{w[+mC]=ActGFP}JMR1 from BDSC.
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View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=GAL4-nos.NGT}40 from BDSC.
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View all literature mentionsDrosophila melanogaster with name w[*]; mael[r20]/TM3, Sb[1] from BDSC.
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View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=PZ}rhi[02086] cn[1]/CyO; ry[506] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23] P{y[+mDint2] w[+mC]=EPgy2}DIP1[EY02625] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=GAL4-nos.NGT}40 from BDSC.
View all literature mentionsDrosophila melanogaster with name In(1)w[m4] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23]; P{y[+mDint2] w[+mC]=EPgy2}EY08366 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=otu-GAL4::VP16.R}1, w[*]; P{w[+mC]=GAL4-nos.NGT}40; P{w[+mC]=GAL4::VP16-nos.UTR}CG6325[MVD1] from BDSC.
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