Myogenic differentiation of human pluripotent stem cells (hPSCs) has been done by gene overexpression or directed differentiation. However, viral integration, long-term culture, and the presence of unwanted cells are the main obstacles. By using CRISPR/Cas9n, a double-reporter human embryonic stem cell (hESC) line was generated for PAX7/MYF5, allowing prospective readout. This strategy allowed pathway screen to define efficient myogenic induction in hPSCs. Next, surface marker screen allowed identification of CD10 and CD24 for purification of myogenic progenitors and exclusion of non-myogenic cells. CD10 expression was also identified on human satellite cells and skeletal muscle progenitors. In vitro and in vivo studies using transgene and/or reporter-free hPSCs further validated myogenic potential of the cells by formation of new fibers expressing human dystrophin as well as donor-derived satellite cells in NSG-mdx4Cv mice. This study provides biological insights for myogenic differentiation of hPSCs using a double-reporter cell resource and defines an improved myogenic differentiation and purification strategy.
Pubmed ID: 30428361 RIS Download
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This unknown targets Rabbit IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets CD10
View all literature mentionsThis monoclonal targets Pax7
View all literature mentionsThis monoclonal targets Human Cell Surface Marker
View all literature mentionsThis monoclonal targets CD24 (Heat Stable Antigen)
View all literature mentionsThis monoclonal targets myosin, sarcomere (MHC)
View all literature mentionsThis polyclonal targets Myf-5 (C-20)
View all literature mentionsThis monoclonal targets Lamin A antibody [EPR4100]
View all literature mentionsThis monoclonal targets CD271 (NGF Receptor)
View all literature mentionsThis monoclonal targets erbB3/HER-3
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Pax7
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Mus musculus with name NOD.Cg-Prkdcscid Il2rg
This polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Pax7
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets Human Cell Surface Marker
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis monoclonal targets erbB3/HER-3
View all literature mentionsThis monoclonal targets myosin, sarcomere (MHC)
View all literature mentions
Mus musculus with name NOD.Cg-Prkdcscid Il2rg
This polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets erbB3/HER-3
View all literature mentionsThis monoclonal targets CD271 (NGF Receptor)
View all literature mentionsThis monoclonal targets CD24 (Heat Stable Antigen)
View all literature mentionsThis monoclonal targets CD10
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis monoclonal targets Pax7
View all literature mentionsThis monoclonal targets Human Cell Surface Marker
View all literature mentionsThis monoclonal targets Lamin A antibody [EPR4100]
View all literature mentionsThis monoclonal targets myosin, sarcomere (MHC)
View all literature mentionsThis polyclonal targets Myf-5 (C-20)
View all literature mentionsThis monoclonal targets Pax7
View all literature mentionsThis monoclonal targets CD271 (NGF Receptor)
View all literature mentionsThis monoclonal targets CD24 (Heat Stable Antigen)
View all literature mentionsThis polyclonal targets Myf-5 (C-20)
View all literature mentionsThis monoclonal targets Pax7
View all literature mentionsThis monoclonal targets CD10
View all literature mentionsThis monoclonal targets Lamin A antibody [EPR4100]
View all literature mentions