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Publication

Microtubule Acetylation Is Required for Mechanosensation in Drosophila.

Cell reports | 2018

At the cellular level, α-tubulin acetylation alters the structure of microtubules to render them mechanically resistant to compressive forces. How this biochemical property of microtubule acetylation relates to mechanosensation remains unknown, although prior studies have shown that microtubule acetylation influences touch perception. Here, we identify the major Drosophila α-tubulin acetylase (dTAT) and show that it plays key roles in several forms of mechanosensation. dTAT is highly expressed in the larval peripheral nervous system (PNS), but it is largely dispensable for neuronal morphogenesis. Mutation of the acetylase gene or the K40 acetylation site in α-tubulin impairs mechanical sensitivity in sensory neurons and behavioral responses to gentle touch, harsh touch, gravity, and vibration stimuli, but not noxious thermal stimulus. Finally, we show that dTAT is required for mechanically induced activation of NOMPC, a microtubule-associated transient receptor potential channel, and functions to maintain integrity of the microtubule cytoskeleton in response to mechanical stimulation.

Pubmed ID: 30355484 RIS Download

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Associated grants

Agency: NIMH NIH HHS, United States
Id: R01 MH115767
Agency: NIGMS NIH HHS, United States
Id: T32 GM008570
Agency: NINDS NIH HHS, United States
Id: R21 NS107924
Agency: NIBIB NIH HHS, United States
Id: P41 EB002025
Agency: NINDS NIH HHS, United States
Id: R21 NS101553
Agency: NIH HHS, United States
Id: P40 OD018537
Agency: NINDS NIH HHS, United States
Id: R01 NS076614
Agency: NINDS NIH HHS, United States
Id: R01 NS089787

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This is a list of tools and resources that we have found mentioned in this publication.

FastQC (tool)

RRID:SCR_014583

Quality control software that perform checks on raw sequence data coming from high throughput sequencing pipelines. This software also provides a modular set of analyses which can give a quick impression of the quality of the data prior to further analysis.

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Bloomington Drosophila Stock Center (tool)

RRID:SCR_006457

Collects, maintains and distributes Drosophila melanogaster strains for research. Emphasis is placed on genetic tools that are useful to a broad range of investigations. These include basic stocks of flies used in genetic analysis such as marker, balancer, mapping, and transposon-tagging strains; mutant alleles of identified genes, including a large set of transposable element insertion alleles; defined sets of deficiencies and a variety of other chromosomal aberrations; engineered lines for somatic and germline clonal analysis; GAL4 and UAS lines for targeted gene expression; enhancer trap and lacZ-reporter strains with defined expression patterns for marking tissues; and a collection of transposon-induced lethal mutations.

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Rabbit Anti-Green Fluorescent Protein (GFP) Polyclonal Antibody, Unconjugated (antibody)

RRID:AB_221569

This polyclonal targets GFP

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Living Colors® DsRed Polyclonal Antibody (antibody)

RRID:AB_10013483

This polyclonal targets DsRed

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futsch antibody - Benzer, S. / Colley, N.; California Institute of Technology (antibody)

RRID:AB_528403

This monoclonal targets futsch

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c-myc (antibody)

RRID:AB_2266850

This monoclonal targets MYC

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Monoclonal Anti-alpha-Tubulin-FITC antibody produced in mouse (antibody)

RRID:AB_476967

This monoclonal targets alpha-Tubulin-FITC antibody produced in mouse

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Acetylated Tubulin (antibody)

RRID:AB_609894

This monoclonal targets derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).

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