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Lipids are structural components of cellular membranes and signaling molecules that are widely involved in development and diseases, but the underlying molecular mechanisms are poorly understood, partly because of the vast variety of lipid species and complexity of synthetic and turnover pathways. From a genetic screen, we identify that mannosyl glucosylceramide (MacCer), a species of glycosphingolipid (GSL), promotes synaptic bouton formation at the Drosophila neuromuscular junction (NMJ). Pharmacological and genetic analysis shows that the NMJ growth-promoting effect of MacCer depends on normal lipid rafts, which are known to be composed of sphingolipids, sterols and select proteins. MacCer positively regulates the synaptic level of Wnt1/Wingless (Wg) and facilitates presynaptic Wg signaling, whose activity is raft-dependent. Furthermore, a functional GSL-binding motif in Wg exhibiting a high affinity for MacCer is required for normal NMJ growth. These findings reveal a novel mechanism whereby the GSL MacCer promotes synaptic bouton formation via Wg signaling.
Pubmed ID: 30355446 RIS Download
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View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
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View all literature mentionsThis monoclonal targets Mouse Drosophila fasciclin II
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View all literature mentionsDrosophila melanogaster with name wg[1] cn[1] from BDSC.
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View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-wg.H.T:HA1}3C from DGGR.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-wg.H.T:HA1}6C from BDSC.
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View all literature mentionsThis monoclonal targets Glutamate receptor subunit, DGluR-IIA
View all literature mentionsThis monoclonal targets Discs large (Drosophila)
View all literature mentionsThis monoclonal targets futsch
View all literature mentionsThis monoclonal targets Mouse Drosophila Wingless protein
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View all literature mentionsDrosophila melanogaster with name wg[1] cn[1] from BDSC.
View all literature mentionsThis monoclonal targets actin
View all literature mentionsThis unknown targets Horseradish Peroxidase
View all literature mentionsThis monoclonal targets Green Fluorescent Protein
View all literature mentionsThis monoclonal targets Myc-tag mAb
View all literature mentionsThis monoclonal targets anti-HA-tag
View all literature mentionsThis monoclonal targets Rab11
View all literature mentionsThis monoclonal targets Mouse Drosophila Frizzled 2
View all literature mentionsThis monoclonal targets Mouse Drosophila syntaxin
View all literature mentionsThis monoclonal targets Mouse Drosophila fasciclin II
View all literature mentionsThis monoclonal targets Glutamate receptor subunit, DGluR-IIA
View all literature mentionsThis monoclonal targets Discs large (Drosophila)
View all literature mentionsThis monoclonal targets futsch
View all literature mentionsThis monoclonal targets Mouse Drosophila Wingless protein
View all literature mentionsThis monoclonal targets cysteine string protein (CSP), Drosophila common
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View all literature mentionsDrosophila melanogaster with name P{w[+mW.hs]=GawB}OK6 from BDSC.
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View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-spin.myc-EGFP}B from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)BSC671, P+PBac{w[+mC]=XP3.WH3}BSC671/TM6C, Sb[1] cu[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23]; P{y[+mDint2] w[BR.E.BR]=SUPor-P}Sk2[KG05894] ry[506] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[67c23] P{w[+mC]=lacW}schlank[G0061] schlank[G0061]/FM7c from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] P{w[+mC]=lacW}schlank[G0489] P{neoFRT}19A/FM7c; P{ey-FLP.N}5 from DGGR.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMC04074}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(2L)Exel7063/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23]; P{w[+mC]=lacW}lace[k05305]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name Adh[n7] lace[2] cn[1] vg[1]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name cn[1] arr[2] bw[1] speck[1]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23]; P{w[+mC]=lacW}arr[k08131]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*] brn[l.6P6]/FM7a from BDSC.
View all literature mentionsDrosophila melanogaster with name brn[fs.107] v[24]/FM3 from BDSC.
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