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Gradients of signaling proteins are essential for inducing tissue morphogenesis. However, mechanisms of gradient formation remain controversial. Here we characterized the distribution of fluorescently-tagged signaling proteins, FGF and FGFR, expressed at physiological levels from the genomic knock-in alleles in Drosophila. FGF produced in the larval wing imaginal-disc moves to the air-sac-primordium (ASP) through FGFR-containing cytonemes that extend from the ASP to contact the wing-disc source. The number of FGF-receiving cytonemes extended by ASP cells decreases gradually with increasing distance from the source, generating a recipient-specific FGF gradient. Acting as a morphogen in the ASP, FGF activates concentration-dependent gene expression, inducing pointed-P1 at higher and cut at lower levels. The transcription-factors Pointed-P1 and Cut antagonize each other and differentially regulate formation of FGFR-containing cytonemes, creating regions with higher-to-lower numbers of FGF-receiving cytonemes. These results reveal a robust mechanism where morphogens self-generate precise tissue-specific gradient contours through feedback regulation of cytoneme-mediated dispersion.
Pubmed ID: 30328809 RIS Download
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An Antibody supplier and subset of ThermoFisher Scientific which provides fluorescence reagents for various experiments and methods.
View all literature mentionsServes Drosophila research community by collecting and distributing DNA clones and vectors; collecting and distributing Drosophila cell lines; developing and testing genomics technologies for use in Drosophila and assisting members of the research community in their use.
View all literature mentionsThis polyclonal targets anti-phospho-ERK1/2 (Thr202/Tyr204) ( p44/42 MAPK) antibody
View all literature mentionsThis monoclonal targets beta-galactosidase
View all literature mentionsThis monoclonal targets Mouse Drosophila Cut protein product
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets Discs large (Drosophila)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets Yan Drosophila protein
View all literature mentionsThis polyclonal targets Fruit fly (Drosophila melanogaster) LAMP1 - Drosophila Lysosome Marker
View all literature mentionsThis polyclonal targets Fruit fly (Drosophila melanogaster) Rab5 - Drosophila Early Endosome Marker
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsSoftware application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.
View all literature mentionsMolecular biology software for visualizing and documenting gene constructs for InFusion cloning, Gibson assembly, restriction cloning, PCR, and mutagenesis.
View all literature mentionsTools for performing statistical computation including: Clinical Research Calculators Probabilities Distributions Frequency Data Proportions Ordinal Data Correlation & Regression t-Tests & Procedures ANOVA ANCOVA
View all literature mentionsSoftware environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.
View all literature mentionsDrosophila melanogaster with name y[1] M{w[+mC]=nos-Cas9.P}ZH-2A w[*] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-mCD8::GFP.L}LL5, P{UAS-mCD8::GFP.L}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-GFP.nls}8 from BDSC.
View all literature mentionsDrosophila melanogaster with name ry[506] P{ry[+t7.2]=PZ}pnt[07825]/TM3, ry[RK] Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-CD4-mIFP-T2A-HO1}attP2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=10XUAS-IVS-mCD8::RFP}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=lexAop-nSyb-spGFP1-10}2, P{w[+mC]=UAS-CD4-spGFP11}2; MKRS/TM6B from BDSC.
View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=PZ}eIF5B[09143], l(3)09143[09143] ry[506]/TM3, ry[RK] Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS02450}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01638}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00308}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS02038}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00924}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01452}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01046}attP2 from BDSC.
View all literature mentionsThis polyclonal targets Fruit fly (Drosophila melanogaster) Rab5 - Drosophila Early Endosome Marker
View all literature mentionsThis monoclonal targets Mouse Drosophila Cut protein product
View all literature mentionsThis monoclonal targets Discs large (Drosophila)
View all literature mentionsDrosophila melanogaster with name y[1] M{w[+mC]=nos-Cas9.P}ZH-2A w[*] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=lexAop-nSyb-spGFP1-10}2, P{w[+mC]=UAS-CD4-spGFP11}2; MKRS/TM6B from BDSC.
View all literature mentionsThis monoclonal targets Yan Drosophila protein
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis polyclonal targets Fruit fly (Drosophila melanogaster) LAMP1 - Drosophila Lysosome Marker
View all literature mentionsThis unknown targets
View all literature mentionsThis polyclonal targets anti-phospho-ERK1/2 (Thr202/Tyr204) ( p44/42 MAPK) antibody
View all literature mentionsThis monoclonal targets beta-galactosidase
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS02038}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01046}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00924}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00308}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-GFP.nls}8 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{y[+t7.7] w[+mC]=UAS-CD4-mIFP-T2A-HO1}attP2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-mCD8::GFP.L}LL5, P{UAS-mCD8::GFP.L}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=10XUAS-IVS-mCD8::RFP}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=PZ}eIF5B[09143], l(3)09143[09143] ry[506]/TM3, ry[RK] Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS02450}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name ry[506] P{ry[+t7.2]=PZ}pnt[07825]/TM3, ry[RK] Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01638}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01452}attP2 from BDSC.
View all literature mentionsMolecular biology software for visualizing and documenting gene constructs for InFusion cloning, Gibson assembly, restriction cloning, PCR, and mutagenesis.
View all literature mentionsSoftware application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsTools for performing statistical computation including: Clinical Research Calculators Probabilities Distributions Frequency Data Proportions Ordinal Data Correlation & Regression t-Tests & Procedures ANOVA ANCOVA
View all literature mentionsSoftware environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
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