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Visual information is detected by the retina and transmitted into the brain by retinal ganglion cells. In rodents, the visual thalamus is a major recipient of retinal ganglion cells axons and is divided into three functionally distinct nuclei: the dorsal lateral geniculate nucleus (dLGN), ventral LGN (vLGN), and intergeniculate leaflet. Despite being densely innervated by retinal input, each nucleus in rodent visual thalamus possesses diverse molecular profiles which underpin their unique circuitry and cytoarchitecture. Here, we combined large-scale unbiased proteomic and transcriptomic analyses to elucidate the molecular expression profiles of the developing mouse dLGN and vLGN. We identified several extracellular matrix proteins as differentially expressed in these regions, particularly constituent molecules of perineuronal nets (PNNs). Remarkably, we discovered at least two types of molecularly distinct Aggrecan-rich PNN populations in vLGN, exhibiting non-overlapping spatial, temporal, and cell-type specific expression patterns. The mechanisms responsible for the formation of these two populations of PNNs also differ as the formation of Cat315+ PNNs (but not WFA+ PNNs) required input from the retina. This study is first to suggest that cell type- and molecularly specific supramolecular assemblies of extracellular matrix may play important roles in the circuitry associated with the subcortical visual system and in the processing of visual information. OPEN SCIENCE BADGES: This article has received a badge for *Open Materials* because it provided all relevant information to reproduce the study in the manuscript. The complete Open Science Disclosure form for this article can be found at the end of the article. More information about the Open Practices badges can be found at https://cos.io/our-services/open-science-badges/. Cover Image for this issue: doi: 10.1111/jnc.14203.
Pubmed ID: 30326149 RIS Download
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View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsSupports research in cellular analysis, genomics, proteomics, and drug discovery. It has merged with Thermo Fisher Scientific. One of several brands under Thermo Fisher Scientific corporation.
View all literature mentionsThis polyclonal targets Sst
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View all literature mentionsThis monoclonal targets Chondroitin Sulfate Proteoglycan Core Protein Epitope clone Cat-315
View all literature mentionsThis polyclonal targets Aggrecan, amino acids 1177-1326
View all literature mentionsThis polyclonal targets Calretinin
View all literature mentionsThis monoclonal targets VGLUT 1
View all literature mentionsThis polyclonal targets VGLUT 2
View all literature mentionsThis polyclonal targets Iba1
View all literature mentionsThis monoclonal targets actin
View all literature mentionsThis polyclonal targets Glutamate Decarboxylase 65 & 67
View all literature mentionsThis monoclonal targets VGlut2
View all literature mentionsThis monoclonal targets VGlut1
View all literature mentionsMus musculus with name STOCK Tg(Aldh1l1-EGFP)OFC789Gsat/Mmucd from MMRRC.
View all literature mentionsMus musculus with name B6J.Cg-Ssttm2.1(cre)Zjh/MwarJ from IMSR.
View all literature mentionsMus musculus with name B6J.129-Atoh7tm1Gla/Mmucd from MMRRC.
View all literature mentionsMus musculus with name B6.Cg-Tg(Gad1-EGFP)3Gfng/J from IMSR.
View all literature mentionsMus musculus with name B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Tg(Thy1-EYFP)15Jrs/J from IMSR.
View all literature mentionsMus musculus with name B6;129P2-Pvalbtm1(cre)Arbr/J from IMSR.
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