Oncogenic transcription factors such as the leukemic fusion protein RUNX1/ETO, which drives t(8;21) acute myeloid leukemia (AML), constitute cancer-specific but highly challenging therapeutic targets. We used epigenomic profiling data for an RNAi screen to interrogate the transcriptional network maintaining t(8;21) AML. This strategy identified Cyclin D2 (CCND2) as a crucial transmitter of RUNX1/ETO-driven leukemic propagation. RUNX1/ETO cooperates with AP-1 to drive CCND2 expression. Knockdown or pharmacological inhibition of CCND2 by an approved drug significantly impairs leukemic expansion of patient-derived AML cells and engraftment in immunodeficient murine hosts. Our data demonstrate that RUNX1/ETO maintains leukemia by promoting cell cycle progression and identifies G1 CCND-CDK complexes as promising therapeutic targets for treatment of RUNX1/ETO-driven AML.
Pubmed ID: 30300583 RIS Download
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Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
View all literature mentionsBioconductor software package for Empirical analysis of Digital Gene Expression data in R. Used for differential expression analysis of RNA-seq and digital gene expression data with biological replication.
View all literature mentionsOpen source software package for statistical programming language R to create plots based on grammar of graphics. Used for data visualization to break up graphs into semantic components such as scales and layers.
View all literature mentionsUltrafast and memory efficient tool for aligning sequencing reads to long reference sequences. Supports gapped, local, and paired end alignment modes. More suited to finding longer, gapped alignments in comparison with original Bowtie method.
View all literature mentionsSoftware performing alignment of high-throughput RNA-seq data. Aligns RNA-seq reads to reference genome using uncompressed suffix arrays.
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Database to store and display somatic mutation information and related details and contains information relating to human cancers. The mutation data and associated information is extracted from the primary literature. In order to provide a consistent view of the data a histology and tissue ontology has been created and all mutations are mapped to a single version of each gene. The data can be queried by tissue, histology or gene and displayed as a graph, as a table or exported in various formats.
Some key features of COSMIC are:
* Contains information on publications, samples and mutations. Includes samples which have been found to be negative for mutations during screening therefore enabling frequency data to be calculated for mutations in different genes in different cancer types.
* Samples entered include benign neoplasms and other benign proliferations, in situ and invasive tumours, recurrences, metastases and cancer cell lines.
Software ultrafast memory efficient tool for aligning sequencing reads. Bowtie is short read aligner.
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on February 28,2023. Software Python package that provides infrastructure to process data from high-throughput sequencing assays. While the main purpose of HTSeq is to allow you to write your own analysis scripts, customized to your needs, there are also a couple of stand-alone scripts for common tasks that can be used without any Python knowledge.
View all literature mentionsSoftware package that implements the remove unwanted variation (RUV) methods for the normalization of RNA-Seq read counts between samples.
View all literature mentionsSoftware repository for R packages related to analysis and comprehension of high throughput genomic data. Uses separate set of commands for installation of packages. Software project based on R programming language that provides tools for analysis and comprehension of high throughput genomic data.
View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsA commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.
View all literature mentionsSoftware R package of functions and classes for the analysis of oligonucleotide arrays manufactured by Affymetrix. Used to process probe level data and for exploratory oligonucleotide array analysis.
View all literature mentionsIn vivo imaging software which facilitates workflow for in vivo optical, X-ray and microCT image acquisition, analysis and data organization.
View all literature mentionsHuman and mouse genome annotation project which aims to identify all gene features in the human genome using computational analysis, manual annotation, and experimental validation.
View all literature mentionsSoftware package for differential gene expression analysis based on the negative binomial distribution. Used for analyzing RNA-seq data for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates.
View all literature mentionsSoftware performing alignment of high-throughput RNA-seq data. Aligns RNA-seq reads to reference genome using uncompressed suffix arrays.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsNSG-HLA-A2/HHD mutant mice are immunodeficient and express human HLA class 1 heavy and light chains. This strain may be useful as a human hematopoietic engraftment host that supports the maturation of human T cells with transplantation http://jaxmice.jax.org/strain/014570.html.
View all literature mentionsMus musculus with name C57BL/6Smoc-Trp53tm2(flox)Smoc from IMSR.
View all literature mentionsThis polyclonal targets Rb (phospho T821) antibody
View all literature mentionsThis polyclonal targets Immunoglobulins
View all literature mentionsThis polyclonal secondary targets Immunoglobulins
View all literature mentionsThis monoclonal targets CDK6
View all literature mentionsThis monoclonal targets beta Actin
View all literature mentionsThis monoclonal targets Clathrin Heavy Chain
View all literature mentionsThis polyclonal targets AML1/RHD Domain (Ab-2) (50-177) Rabbit pAb
View all literature mentionsThis monoclonal targets Retinoblastoma Protein
View all literature mentionsThis polyclonal targets Cyclin D2
View all literature mentionsThis monoclonal targets E2F4
View all literature mentionsThis monoclonal targets Rb (phospho T826)
View all literature mentionsThis polyclonal targets Phospho-Rb (Ser780)
View all literature mentionsCell line MS-5 [Mouse bone marrow] is a Stromal cell line with a species of origin Mus musculus
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MV4-11 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line THP-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line OCI-AML-3 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HL-60 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SKNO-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line Kasumi-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MV4-11 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SKNO-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis polyclonal targets Immunoglobulins
View all literature mentionsThis polyclonal secondary targets Immunoglobulins
View all literature mentionsThis monoclonal targets CDK6
View all literature mentionsThis monoclonal targets beta Actin
View all literature mentionsThis monoclonal targets Clathrin Heavy Chain
View all literature mentionsThis polyclonal targets AML1/RHD Domain (Ab-2) (50-177) Rabbit pAb
View all literature mentionsThis monoclonal targets Retinoblastoma Protein
View all literature mentionsThis polyclonal targets Cyclin D2
View all literature mentionsThis monoclonal targets E2F4
View all literature mentionsThis monoclonal targets Rb (phospho T826)
View all literature mentionsThis polyclonal targets Phospho-Rb (Ser780)
View all literature mentionsCell line MV4-11 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SKNO-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis polyclonal targets Immunoglobulins
View all literature mentionsThis polyclonal secondary targets Immunoglobulins
View all literature mentionsThis monoclonal targets CDK6
View all literature mentionsThis monoclonal targets beta Actin
View all literature mentionsThis monoclonal targets Clathrin Heavy Chain
View all literature mentionsThis polyclonal targets AML1/RHD Domain (Ab-2) (50-177) Rabbit pAb
View all literature mentionsThis monoclonal targets Retinoblastoma Protein
View all literature mentionsThis polyclonal targets Cyclin D2
View all literature mentionsThis monoclonal targets Rb (phospho T826)
View all literature mentionsThis polyclonal targets Phospho-Rb (Ser780)
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MS-5 [Mouse bone marrow] is a Stromal cell line with a species of origin Mus musculus
View all literature mentionsCell line HL-60 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line THP-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line Kasumi-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis polyclonal targets Phospho-Rb (Ser780)
View all literature mentionsThis monoclonal targets E2F4
View all literature mentionsThis polyclonal targets AML1/RHD Domain (Ab-2) (50-177) Rabbit pAb
View all literature mentionsThis monoclonal targets Retinoblastoma Protein
View all literature mentionsCell line MS-5 [Mouse bone marrow] is a Stromal cell line with a species of origin Mus musculus
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line THP-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HL-60 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line Kasumi-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line Kasumi-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MS-5 [Mouse bone marrow] is a Stromal cell line with a species of origin Mus musculus
View all literature mentionsThis polyclonal secondary targets Immunoglobulins
View all literature mentionsThis monoclonal targets Clathrin Heavy Chain
View all literature mentionsThis monoclonal targets CDK6
View all literature mentionsThis monoclonal targets beta Actin
View all literature mentionsThis polyclonal targets Immunoglobulins
View all literature mentionsThis monoclonal targets Rb (phospho T826)
View all literature mentionsThis polyclonal targets Cyclin D2
View all literature mentionsCell line MV4-11 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HL-60 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SKNO-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line THP-1 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentions