Pyramidal neuron dendrites integrate synaptic input from multiple partners. Different inputs converging on the same dendrite have distinct structural and functional features, but the molecular mechanisms organizing input-specific properties are poorly understood. We identify the orphan receptor GPR158 as a binding partner for the heparan sulfate proteoglycan (HSPG) glypican 4 (GPC4). GPC4 is enriched on hippocampal granule cell axons (mossy fibers), whereas postsynaptic GPR158 is restricted to the proximal segment of CA3 apical dendrites receiving mossy fiber input. GPR158-induced presynaptic differentiation in contacting axons requires cell-surface GPC4 and the co-receptor LAR. Loss of GPR158 increases mossy fiber synapse density but disrupts bouton morphology, impairs ultrastructural organization of active zone and postsynaptic density, and reduces synaptic strength of this connection, while adjacent inputs on the same dendrite are unaffected. Our work identifies an input-specific HSPG-GPR158 interaction that selectively organizes synaptic architecture and function of developing mossy fiber-CA3 synapses in the hippocampus.
Pubmed ID: 30290982 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
A software for (scanning) transmission electron microscope experimental control and analysis.
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Human IgG, Fc fragment
View all literature mentionsThis unknown targets Guinea Pig IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Chicken IgG (H+L)
View all literature mentionsThis monoclonal targets Lsamp (Lamp)
View all literature mentionsThis polyclonal targets VGLUT1
View all literature mentionsThis polyclonal targets Synaptoporin
View all literature mentionsThis monoclonal targets Synaptophysin
View all literature mentionsThis polyclonal targets beta III Tubulin
View all literature mentionsThis monoclonal targets & x03B2;-Galactosidase
View all literature mentionsThis polyclonal targets Glypican 4
View all literature mentionsThis unknown targets Glypican 4, aa 88-101
View all literature mentionsThis polyclonal targets Bassoon
View all literature mentionsThis monoclonal targets PSD-95
View all literature mentionsThis polyclonal targets GPR158
View all literature mentionsThis polyclonal targets GPR158 N-Terminal antibody produced in rabbit
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis polyclonal targets Synapsin I
View all literature mentionsThis monoclonal targets c-Myc
View all literature mentionsThis monoclonal targets HA.11
View all literature mentionsThis polyclonal targets GFP-1010
View all literature mentionsgene symbol note: G protein-coupled receptor 158 mutant strain targeted mutation 1, Velocigene This is a legacy resource.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentionsImaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsMicroscope imaging software suite used with Nikon products. NIS-Elements includes software applications for advanced and standard research, documentation, confocal microscopy, and high-content analysis.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsCell line HEK293T/17 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsgene symbol note: G protein-coupled receptor 158 mutant strain targeted mutation 1, Velocigene This is a legacy resource.
View all literature mentions