Searching across hundreds of databases
The mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) is a master regulator of cell growth and metabolism. Leucine (Leu) activates mTORC1 and many have tried to identify the mechanisms whereby cells sense Leu in this context. Here we describe that the Leu metabolite acetyl-coenzyme A (AcCoA) positively regulates mTORC1 activity by EP300-mediated acetylation of the mTORC1 regulator, Raptor, at K1097. Leu metabolism and consequent mTORC1 activity are regulated by intermediary enzymes. As AcCoA is a Leu metabolite, this process directly correlates with Leu abundance, and does not require Leu sensing via intermediary proteins, as has been described previously. Importantly, we describe that this pathway regulates mTORC1 in a cell-type-specific manner. Finally, we observed decreased acetylated Raptor, and inhibited mTORC1 and EP300 activity in fasted mice tissues. These results provide a direct mechanism for mTORC1 regulation by Leu metabolism.
Pubmed ID: 30197302 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
View all literature mentionsA division of Applied Biosystems selling products for the isolation, detection, quantification, amplification, and characterization of RNA.
View all literature mentionsAn Antibody supplier and subset of ThermoFisher Scientific which provides fluorescence reagents for various experiments and methods.
View all literature mentionsSoftware application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsCell line SH-SY5Y is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MCF-10A is a Spontaneously immortalized cell line with a species of origin Homo sapiens
View all literature mentionsCell line SH-SY5Y is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis polyclonal targets Full length recombinant GFP
View all literature mentionsThis polyclonal targets Phospho-ULK1 (Ser757)
View all literature mentionsThis polyclonal targets ULK1
View all literature mentionsThis unknown targets mTOR, phospho (Ser2481)
View all literature mentionsThis unknown targets mTOR
View all literature mentionsThis monoclonal targets α-Tubulin
View all literature mentionsThis monoclonal targets S6 Ribosomal Protein, phospho (Ser235 / Ser236)
View all literature mentionsThis monoclonal targets S6 Ribosomal Protein
View all literature mentionsThis monoclonal targets RagC
View all literature mentionsThis monoclonal targets RagB (D18F3) Rabbit mAb
View all literature mentionsThis monoclonal targets RagA (D8B5) Rabbit mAb
View all literature mentionsThis polyclonal targets Phospho-Raptor (Ser792)
View all literature mentionsThis monoclonal targets Raptor (24C12) Rabbit mAb
View all literature mentionsThis polyclonal targets KIAA1303
View all literature mentionsThis polyclonal targets PRKCA
View all literature mentionsThis monoclonal targets PRAS40
View all literature mentionsThis monoclonal targets p70 S6 Kinase, phospho (Thr389)
View all literature mentionsThis polyclonal targets p70 S6 Kinase
View all literature mentionsThis monoclonal targets c-myc; Mouse monoclonal antibody (clone 9E10) IgG1 & 954;
View all literature mentionsThis monoclonal targets LAMTOR1/C11orf59 (D11H6) XP Rabbit mAb
View all literature mentionsThis monoclonal targets G L (86B8) Rabbit mAb
View all literature mentionsThis monoclonal targets LAMP1
View all literature mentionsThis polyclonal targets Histone H3
View all literature mentionsThis unknown targets HA Epitope Tag
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets GAPDH antibody [6C5]
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets p300 CT
View all literature mentionsThis polyclonal targets 4E-BP1
View all literature mentionsThis polyclonal targets Acetylated-Lysine
View all literature mentionsThis monoclonal targets Acetylated-Lysine (Ac-K2-100) Rabbit mAb
View all literature mentionsThis polyclonal targets Phospho-ATP-Citrate Lyase (Ser455)
View all literature mentionsThis polyclonal targets Acly
View all literature mentionsThis monoclonal targets Atg7 (D12B11) Rabbit mAb
View all literature mentionsThis monoclonal targets Phospho-Akt (Ser473)
View all literature mentionsThis polyclonal targets Actin
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentions3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 28,2023. Software application which can analyze basic Western blot data and export them for use in spreadsheet programs. Users can create standardized or custom lab reports and can share them with colleagues. Image Studio Lite has been discontinued and replaced with Empiria Studio Software.
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsCell line MCF-7 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsThis polyclonal targets Actin
View all literature mentionsThis polyclonal targets KIAA1303
View all literature mentionsThis polyclonal targets Full length recombinant GFP
View all literature mentionsThis monoclonal targets p300 CT
View all literature mentionsThis monoclonal targets GAPDH antibody [6C5]
View all literature mentionsThis polyclonal targets Acetylated-Lysine
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis polyclonal targets 4E-BP1
View all literature mentionsThis monoclonal targets Acetylated-Lysine (Ac-K2-100) Rabbit mAb
View all literature mentionsThis polyclonal targets Phospho-ATP-Citrate Lyase (Ser455)
View all literature mentionsThis monoclonal targets Atg7 (D12B11) Rabbit mAb
View all literature mentionsThis polyclonal targets Acly
View all literature mentionsThis monoclonal targets Phospho-Akt (Ser473)
View all literature mentionsThis monoclonal targets p70 S6 Kinase, phospho (Thr389)
View all literature mentionsThis monoclonal targets c-myc; Mouse monoclonal antibody (clone 9E10) IgG1 & 954;
View all literature mentionsThis polyclonal targets p70 S6 Kinase
View all literature mentionsThis monoclonal targets PRAS40
View all literature mentionsThis monoclonal targets LAMP1
View all literature mentionsThis monoclonal targets G L (86B8) Rabbit mAb
View all literature mentionsThis polyclonal targets Histone H3
View all literature mentionsThis monoclonal targets LAMTOR1/C11orf59 (D11H6) XP Rabbit mAb
View all literature mentionsThis unknown targets HA Epitope Tag
View all literature mentionsThis unknown targets
View all literature mentionsThis polyclonal targets ULK1
View all literature mentionsThis monoclonal targets Raptor (24C12) Rabbit mAb
View all literature mentionsThis unknown targets mTOR
View all literature mentionsThis polyclonal targets Phospho-ULK1 (Ser757)
View all literature mentionsThis monoclonal targets α-Tubulin
View all literature mentionsThis monoclonal targets S6 Ribosomal Protein
View all literature mentionsThis monoclonal targets S6 Ribosomal Protein, phospho (Ser235 / Ser236)
View all literature mentionsCell line MCF-7 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsThis unknown targets mTOR, phospho (Ser2481)
View all literature mentionsThis monoclonal targets RagA (D8B5) Rabbit mAb
View all literature mentionsThis polyclonal targets PRKCA
View all literature mentionsThis polyclonal targets Phospho-Raptor (Ser792)
View all literature mentionsThis monoclonal targets RagC
View all literature mentionsThis monoclonal targets RagB (D18F3) Rabbit mAb
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
