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Publication

Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone Marrow.

Cell reports | 2018

Neutrophils are short-lived cells that play important roles in both health and disease. Neutrophils and monocytes originate from the granulocyte monocyte progenitor (GMP) in bone marrow; however, unipotent neutrophil progenitors are not well defined. Here, we use cytometry by time of flight (CyTOF) and single-cell RNA sequencing (scRNA-seq) methodologies to identify a committed unipotent early-stage neutrophil progenitor (NeP) in adult mouse bone marrow. Importantly, we found a similar unipotent NeP (hNeP) in human bone marrow. Both NeP and hNeP generate only neutrophils. NeP and hNeP both significantly increase tumor growth when transferred into murine cancer models, including a humanized mouse model. hNeP are present in the blood of treatment-naive melanoma patients but not of healthy subjects. hNeP can be readily identified by flow cytometry and could be used as a biomarker for early cancer discovery. Understanding the biology of hNeP should allow the development of new therapeutic targets for neutrophil-related diseases, including cancer.

Pubmed ID: 30157427 RIS Download

Research resources used in this publication

Additional research tools detected in this publication

None found

Antibodies used in this publication

Associated grants

  • Agency: NCI NIH HHS, United States
    Id: U01 CA224766
  • Agency: NCI NIH HHS, United States
    Id: P30 CA168524
  • Agency: NHLBI NIH HHS, United States
    Id: P01 HL136275
  • Agency: NCI NIH HHS, United States
    Id: R01 CA202987
  • Agency: NIAID NIH HHS, United States
    Id: T32 AI125179
  • Agency: NHLBI NIH HHS, United States
    Id: R01 HL134236
  • Agency: NIH HHS, United States
    Id: S10 OD018499

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

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PE-CF594 Rat Anti-Mouse Siglec-F Antibody (antibody)

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NOD.Cg-Prkdcscid Il2rg Tg(CMV-IL3.CSF2.KITLG)1Eav/MloySzJ (organism)

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