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Guanine nucleotide exchange factors (GEFs) play important roles in many cellular processes, including regulation of the structural plasticity of dendritic spines. A GEF protein, adenomatous polyposis coli-stimulated GEF 1 (Asef1, ARHGEF4) is highly expressed in the nervous system. However, the function of Asef1 has not been investigated in neurons. Here, we present evidence showing that Asef1 negatively regulates the synaptic localization of postsynaptic density protein 95 (PSD-95) in the excitatory synapse by inhibiting Staufen-mediated synaptic localization of PSD-95. Accordingly, Asef1 expression impairs synaptic transmission in hippocampal cultured neurons. In addition, neuronal activity facilitates the dissociation of Asef1 from Staufen in a phosphoinositide 3 kinase (PI3K)-dependent manner. Taken together, our data reveal Asef1 functions as a negative regulator of synaptic localization of PSD-95 and synaptic transmission.
Pubmed ID: 30125942 RIS Download
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This polyclonal targets Asef (N-20)
View all literature mentionsThis unknown targets Staufen
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets HA
View all literature mentionsThis monoclonal targets PSD-95
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis monoclonal targets Homer 1
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets AMPA-Selective Glutamate Receptor 1 (GluR1)
View all literature mentionsThis polyclonal targets Synapsin I
View all literature mentionsThis monoclonal targets c-Myc
View all literature mentionsThis polyclonal targets Asef (N-20)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets AMPA-Selective Glutamate Receptor 1 (GluR1)
View all literature mentionsThis monoclonal targets Homer 1
View all literature mentionsThis polyclonal targets Synapsin I
View all literature mentionsThis monoclonal targets PSD-95
View all literature mentionsThis unknown targets Staufen
View all literature mentionsThis monoclonal targets HA
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets c-Myc
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