Searching across hundreds of databases
Bone marrow (BM) perivascular stromal cells and vascular endothelial cells (ECs) are essential for hematopoietic stem cell (HSC) maintenance, but the roles of distinct niche compartments during HSC regeneration are less understood. Here we show that Leptin receptor-expressing (LepR+) BM stromal cells and ECs dichotomously regulate HSC maintenance and regeneration via secretion of pleiotrophin (PTN). BM stromal cells are the key source of PTN during steady-state hematopoiesis because its deletion from stromal cells, but not hematopoietic cells, osteoblasts, or ECs, depletes the HSC pool. Following myelosuppressive irradiation, PTN expression is increased in bone marrow endothelial cells (BMECs), and PTN+ ECs are more frequent in the niche. Moreover, deleting Ptn from ECs impairs HSC regeneration whereas Ptn deletion from BM stromal cells does not. These findings reveal dichotomous and complementary regulation of HSC maintenance and regeneration by BM stromal cells and ECs.
Pubmed ID: 30100167 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This polyclonal targets Perilipin
View all literature mentionsThis unknown targets CXCL12 / SDF-1
View all literature mentionsThis monoclonal targets CD140a
View all literature mentionsThis monoclonal targets Rat NG2
View all literature mentionsThis polyclonal targets Rabbit Endomucin
View all literature mentionsThis polyclonal targets Mouse/Rat CD31/PECAM-1 Alexa Fluor 488 Affinity Purified PAb
View all literature mentionsThis monoclonal targets CD31
View all literature mentionsThis polyclonal targets Mouse Osteopontin (OPN) Affinity Purified Ab
View all literature mentionsThis polyclonal secondary targets IgY (H+L)
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis unknown targets Rat IgG2a kappa Isotype Control Alexa Fluor?? 647 Clone R35-95
View all literature mentionsThis monoclonal targets Rat IgG2b k
View all literature mentionsThis unknown targets Rat IgG2a Clone: R35-95 R-Phycoerythrin /Tagged
View all literature mentionsThis unknown targets Isotype Control
View all literature mentionsThis unknown targets Rat IgG1 kappa Isotype Control FITC Clone R3-34
View all literature mentionsThis unknown targets Rat IgG2a kappa Isotype Control APC-Cy7 Clone R35-95
View all literature mentionsThis monoclonal targets Rat IgG2b k
View all literature mentionsThis monoclonal targets CD34
View all literature mentionsThis monoclonal targets Mouse CD135
View all literature mentionsThis unknown targets Lineage Cocktail with Isotype Control
View all literature mentionsThis monoclonal targets Mouse Ly-6A / E
View all literature mentionsThis monoclonal targets CD117 (c-Kit)
View all literature mentionsThis monoclonal targets CD41
View all literature mentionsThis unknown targets Mouse CD45
View all literature mentionsThis monoclonal targets Rat IgG2b k
View all literature mentionsThis monoclonal targets CD3 Molecular Complex
View all literature mentionsThis monoclonal targets TER-119/Erythroid cells
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets Ly-6G and Ly-6C
View all literature mentionsThis monoclonal targets Mouse CD45.2
View all literature mentionsThis monoclonal targets CD45.2
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD45R (B220)
View all literature mentionsThis polyclonal targets Rabbit Leptin receptor(L/M/S) FITC
View all literature mentionsThis monoclonal targets CD144
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Commd10Tg(Vav1-icre)A2Kio/J from IMSR.
View all literature mentionsMus musculus with name B6.FVB-Tg(Cdh5-cre)7Mlia/J from IMSR.
View all literature mentionsMus musculus with name B6.129(Cg)-Leprtm2(cre)Rck/J from IMSR.
View all literature mentionsMus musculus with name B6.FVB-Tg(BGLAP-cre)1Clem/J from IMSR.
View all literature mentions
Mus musculus with name B6.SJL-Ptprca Pepc
Mus musculus with name C57BL/6J from IMSR.
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
