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Publication

Differential HDAC6 Activity Modulates Ciliogenesis and Subsequent Mechanosensing of Endothelial Cells Derived from Pluripotent Stem Cells.

Cell reports | 2018

The role of primary cilia in mechanosensation is essential in endothelial cell (EC) shear responsiveness. Here, we find that venous, capillary, and progenitor ECs respond to shear stress in vitro in a cilia-dependent manner. We then demonstrate that primary cilia assembly in human induced pluripotent stem cell (hiPSC)-derived ECs varies between different cell lines with marginal influence of differentiation protocol. hiPSC-derived ECs lacking cilia do not align to shear stress, lack stress fiber assembly, have uncoordinated migration during wound closure in vitro, and have aberrant calcium influx upon shear exposure. Transcriptional analysis reveals variation in regulatory genes involved in ciliogenesis among different hiPSC-derived ECs. Moreover, inhibition of histone deacetylase 6 (HDAC6) activity in hiPSC-ECs lacking cilia rescues cilia formation and restores mechanical sensing. Taken together, these results show the importance of primary cilia in hiPSC-EC mechano-responsiveness and its modulation through HDAC6 activity varies among hiPSC-ECs.

Pubmed ID: 30044986 RIS Download

Research resources used in this publication

Mouse Anti-CD31 Monoclonal Antibody, Phycoerythrin Conjugated, Clone WM59 (RRID:AB_395839) (antibody)

Additional research tools detected in this publication

None found

Antibodies used in this publication

Associated grants

Agency: NHLBI NIH HHS, United States
Id: F31 HL134329
Agency: NHLBI NIH HHS, United States
Id: F32 HL128038
Agency: NIH HHS, United States
Id: S10 OD016230
Agency: NCI NIH HHS, United States
Id: U54 CA210173

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