COMMD5/HCaRG is involved in tissue repair, and its low expression is associated with tumorigenicity. Cell growth, migration, and differentiation are controlled by COMMD5. We previously reported that COMMD5 inhibited the growth of renal carcinoma cells by regulating expression or phosphorylation of ErbB members. Here, we demonstrate that COMMD5 is crucial for the stability of the cytoskeleton. Its silencing leads to a major re-organization of actin and microtubule networks. The N terminus of COMMD5 binds to the endosomal Rab5, and its C terminus, including the COMMD domain, binds to the cytoskeletal scaffolding. COMMD5 participates in long-range endosome transport, including epidermal growth factor receptor (EGFR) recycling, and provides the strength to deform and assist the scission of vesicles into sorting endosomes. This study establishes the molecular mechanism by which COMMD5 acts as an adaptor protein to coordinate endosomal trafficking and reveals its important role for EGFR transport and activity.
Pubmed ID: 30021164 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This monoclonal targets Rab5
View all literature mentionsThis polyclonal targets ANXA3
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets Nup98
View all literature mentionsThis polyclonal targets ARPC4
View all literature mentionsThis monoclonal targets E-cadherin
View all literature mentionsThis polyclonal targets Neu (C-18)
View all literature mentionsThis recombinant monoclonal targets Insulin Receptor beta
View all literature mentionsThis monoclonal targets Human EEA1 - Early Endosome Marker
View all literature mentionsThis polyclonal targets beta Tubulin antibody - Loading Control
View all literature mentionsThis monoclonal targets beta-Tubulin
View all literature mentionsThis polyclonal targets Phospho-Akt (Ser473)
View all literature mentionsThis monoclonal targets EGFR
View all literature mentionsThis unknown targets EGF Receptor
View all literature mentionsThis polyclonal targets EGFR
View all literature mentionsThis unknown targets Phospho-EGFR (Tyr1086)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets Mouse IgG
View all literature mentionsThis polyclonal targets goat anti-rabbit IgG-HRP
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsThis polyclonal targets Beta-Tubulin
View all literature mentionsThis monoclonal targets GFP
View all literature mentionsThis polyclonal targets COMMD1
View all literature mentionsThis polyclonal targets COMMD5
View all literature mentionsThis polyclonal targets alpha Tubulin - Loading Control
View all literature mentionsThis polyclonal targets Actin
View all literature mentionsCell line HK-2 [Human kidney] is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HEK293 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis monoclonal targets Rab5
View all literature mentions