The diversity of cell types and regulatory states in the brain, and how these change during aging, remains largely unknown. We present a single-cell transcriptome atlas of the entire adult Drosophila melanogaster brain sampled across its lifespan. Cell clustering identified 87 initial cell clusters that are further subclustered and validated by targeted cell-sorting. Our data show high granularity and identify a wide range of cell types. Gene network analyses using SCENIC revealed regulatory heterogeneity linked to energy consumption. During aging, RNA content declines exponentially without affecting neuronal identity in old brains. This single-cell brain atlas covers nearly all cells in the normal brain and provides the tools to study cellular diversity alongside other Drosophila and mammalian single-cell datasets in our unique single-cell analysis platform: SCope (http://scope.aertslab.org). These results, together with SCope, allow comprehensive exploration of all transcriptional states of an entire aging brain.
Pubmed ID: 29909982 RIS Download
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View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-MitoTimer}3 from BDSC.
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View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-mCD8::GFP.L}LL5, P{UAS-mCD8::GFP.L}2 from BDSC.
View all literature mentionsDiscontinued
View all literature mentionsDrosophila melanogaster with name w[1118]; PBac{y[+mDint2] w[+mC]=pros-GFP.FPTB}VK00037 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mW.hs]=GawB}GH146 from BDSC.
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View all literature mentionsDrosophila melanogaster with name y[1] w[*] Mi{PT-GFSTF.2}Imp[MI05901-GFSTF.2] from BDSC.
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View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
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View all literature mentionsscikit-learn: machine learning in Python
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View all literature mentionsThis polyclonal secondary targets IgG (H+L)
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View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
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View all literature mentionsThis monoclonal targets Pigment-dispersing factor neuropeptide
View all literature mentionsThis monoclonal targets elav Drosophila protein; embryonic lethal abnormal vision
View all literature mentionsThis monoclonal targets Prospero protein
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View all literature mentionsCommand-line software tools for processing biological sequencing data. Barcode demultiplexing, adapter trimming, etc. Primarily written to support an Illumina based pipeline - but should work with any FASTQs.
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View all literature mentionsscikit-learn: machine learning in Python
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View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis monoclonal targets Bruchpilot
View all literature mentionsA software tool which provides interactive visualization capability for the integrated analysis of high-dimensional gene expression data.
View all literature mentionsThis monoclonal targets Pigment-dispersing factor neuropeptide
View all literature mentionsThis monoclonal targets elav Drosophila protein; embryonic lethal abnormal vision
View all literature mentionsThis monoclonal targets Prospero protein
View all literature mentionsThis monoclonal targets Pigment-dispersing factor neuropeptide
View all literature mentionsSoftware performing alignment of high-throughput RNA-seq data. Aligns RNA-seq reads to reference genome using uncompressed suffix arrays.
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View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
View all literature mentionsDrosophila melanogaster with name DGRP-551 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=UAS-mCD8::GFP.L}LL5, P{UAS-mCD8::GFP.L}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mW.hs]=GawB}VGlut[OK371] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=GawB}c767 from BDSC.
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View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mW.hs]=GawB}GH146 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-MitoTimer}3 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; PBac{y[+mDint2] w[+mC]=pros-GFP.FPTB}VK00037 from BDSC.
View all literature mentions