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Activation of GPR55 increases neural stem cell proliferation and promotes early adult hippocampal neurogenesis.

British journal of pharmacology | 2018

The cannabinoid system exerts functional regulation of neural stem cell (NSC) proliferation and adult neurogenesis, yet not all effects of cannabinoid-like compounds seen can be attributed to the cannabinoid 1 (CB1 ) or CB2 receptor. The recently de-orphaned GPR55 has been shown to be activated by numerous cannabinoid ligands suggesting that GPR55 is a third cannabinoid receptor. Here, we examined the role of GPR55 activation in NSC proliferation and early adult neurogenesis.

Pubmed ID: 29888782 RIS Download

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Associated grants

  • Agency: NIDA NIH HHS, United States
    Id: P30 DA013429
  • Agency: NIAAA NIH HHS, United States
    Id: U01 AA023552
  • Agency: NIDA NIH HHS, United States
    Id: T32 DA007237
  • Agency: NIAAA NIH HHS, United States
    Id: R37 AA015913

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IUPHAR/BPS Guide to Pharmacology (tool)

RRID:SCR_013077

Portal and searchable database of pharmacological information. Information is presented at two levels, the initial view or landing pages for each target family provide expert-curated overviews of the key properties and the available selective ligands and tool compounds. For selected targets, more detailed introductory chapters for each family are available along with curated information on the pharmacological, physiological, structural, genetic and pathophysiogical properties of each target.

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FITC BrdU Flow Kit (antibody)

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Nestin (antibody)

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GraphPad Prism (software resource)

RRID:SCR_002798

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RRID:CVCL_E921

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FlowJo (software resource)

RRID:SCR_008520

Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.

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Sox2 (antibody)

RRID:AB_10712763

This monoclonal targets Sox2

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Ki67 antibody - Proliferation Marker (antibody)

RRID:AB_443209

This polyclonal targets Ki67 antibody - Proliferation Marker

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FITC BrdU Flow Kit (antibody)

RRID:AB_2617060

This monoclonal targets Bromodeoxyuridine

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3-Tubulin (D71G9) XP Rabbit mAb (antibody)

RRID:AB_10694505

This monoclonal targets 3-Tubulin

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GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

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