The differentiation of the lateral plate mesoderm cells into heart field cells constitutes a critical step in the development of cardiac tissue and the genesis of functional cardiomyocytes. Hippo signaling controls cardiomyocyte proliferation, but the role of Hippo signaling during early cardiogenesis remains unclear. Here, we show that Hippo signaling regulates atrial cell number by specifying the developmental potential of cells within the anterior lateral plate mesoderm (ALPM), which are incorporated into the venous pole of the heart tube and ultimately into the atrium of the heart. We demonstrate that Hippo signaling acts through large tumor suppressor kinase 1/2 to modulate BMP signaling and the expression of hand2, a key transcription factor that is involved in the differentiation of atrial cardiomyocytes. Collectively, these results demonstrate that Hippo signaling defines venous pole cardiomyocyte number by modulating both the number and the identity of the ALPM cells that will populate the atrium of the heart.
Pubmed ID: 29809141 RIS Download
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Commercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.
View all literature mentionsA commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.
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View all literature mentionsImaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
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View all literature mentionsThis web site has been created to provide a centralized source for CS2 and related vector information. It will be updated (on an irregular basis) with additional information, including new vector designs. CS2 vector: pCS2 is a multipurpose expression vector. Although originally designed for expressing proteins in Xenopus embryos from either injected RNA or DNA, pCS2 is also useful for high-level transient expression in a wide variety of mammalian and avian cells. It is also functional in zebrafish embryos (as DNA or RNA), and it can be used for in vitro transcription/translation (using, for example, the Promega TnT system). A number of derivatives of CS2 have been constructed that allow fusions to epitope tags and other marker proteins, as well as nuclear localization signals or the gal4 DNA binding and activation domains. In almost all cases, the same reading frames are used for the fusion vectors, to facilitate moving genes between multiple CS2 derivatives. pCS2 features: pCS2 contains a strong enhancer/promoter (simian CMV IE94) followed by a polylinker and the SV40 late polyadenlyation site. An SP6 promoter is present in the 5'' untranslated region of the mRNA from the sCMV promoter, allowing in vitro RNA synthesis of sequences cloned into the polylinker. A T7 promoter in reverse orientation between the polylinker and the SV40 polyA site for probe synthesis, as well as a second polylinker after the SV40 polyA site to provide several possible sites to linearize the vector for SP6 RNA transcription. The vector backbone is from pBluescript II KS and includes the amp resistance gene and an f1 origin for producing single stranded DNA. :Sponsors: This resource is supported by the University of Michigan. : Keywords: Vector, Resource, Expression, Protein, Xenopus, Embryo, RNA, DNA, Transient, Zebrafish, Domain, mRNA, Synthesis, Promoter,
View all literature mentionsDanio rerio with name allele name: mw29Tg from ZFIN.
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View all literature mentionsThis monoclonal targets Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467)
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