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Neuropeptide Y (NPY) is a peptide neurotransmitter abundantly expressed in the mammalian retina. Since its discovery, NPY has been studied in retinas of several species, but detailed characterization of morphology, cell-type, and connectivity has never been conducted in larger mammals including humans and pigs. As the pig due to size and cellular composition is a well-suited animal for retinal research, we chose to compare the endogenous NPY system of the human retina to that of pigs to support future research in this field. In the present study, using immunohistochemistry, confocal microscopy and 3D reconstructions, we found NPY to be expressed in GABAergic and calretinin-immunoreactive (-ir) amacrine cells of both species as well as parvalbumin-ir amacrine cells of humans. Furthermore, we identified at least two different types of medium- to wide-field NPY-ir amacrine cells. Finally, we detected likely synaptic appositions between the NPY-ir amacrine cells and melanopsin- and nonmelanopsin-ir ganglion cells, GABAergic and dopaminergic amacrine cells, rod bipolar cells, and horizontal cells, suggesting that NPY-ir cells play diverse roles in modulation of both image and non-image forming retinal signaling. These findings extend existing knowledge on NPY and NPY-expressing cells in the human and porcine retina showing a high degree of comparability. The extensive distribution and connectivity of NPY-ir cells described in the present study further highlights the potential importance of NPY signaling in retinal function.
Pubmed ID: 29665009 RIS Download
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This polyclonal targets Brn-3a (C-20)
View all literature mentionsThis unknown targets Glutamic Acid Decarboxylase 65, aa 5-22
View all literature mentionsThis unknown targets Human/primate melanopsin, C-terminal melanopsin
View all literature mentionsThis monoclonal targets Choline Acetyltransferase
View all literature mentionsThis polyclonal targets Neuropeptide Y (NPY) antibody produced in rabbit
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View all literature mentionsThis unknown targets Guinea Pig IgG (H+L)
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View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsImaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsThis polyclonal targets Neuropeptide Y (NPY) antibody produced in rabbit
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase
View all literature mentionsThis polyclonal targets RBPMS
View all literature mentionsThis unknown targets Protein Kinase Calpha
View all literature mentionsThis monoclonal targets Protein Kinase C (PKC)
View all literature mentionsThis polyclonal targets Rabbit NeuN (polyclonal)
View all literature mentionsThis unknown targets Human/primate melanopsin, C-terminal melanopsin
View all literature mentionsThis unknown targets Glutamic Acid Decarboxylase 65, aa 5-22
View all literature mentionsThis monoclonal targets Choline Acetyltransferase
View all literature mentionsThis monoclonal targets Calbindin D-28k
View all literature mentionsThis polyclonal targets Brn-3a (C-20)
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