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Screens for genes that orchestrate neural circuit formation in mammals have been hindered by practical constraints of germline mutagenesis. To overcome these limitations, we combined RNA-seq with somatic CRISPR mutagenesis to study synapse development in the mouse retina. Here synapses occur between cellular layers, forming two multilayered neuropils. The outer neuropil, the outer plexiform layer (OPL), contains synapses made by rod and cone photoreceptor axons on rod and cone bipolar dendrites, respectively. We used RNA-seq to identify selectively expressed genes encoding cell surface and secreted proteins and CRISPR-Cas9 electroporation with cell-specific promoters to assess their roles in OPL development. Among the genes identified in this way are Wnt5a and Wnt5b. They are produced by rod bipolars and activate a non-canonical signaling pathway in rods to regulate early OPL patterning. The approach we use here can be applied to other parts of the brain.
Pubmed ID: 29576390 RIS Download
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This polyclonal targets DsRed
View all literature mentionsThis polyclonal targets MAP2 - Neuronal Marker
View all literature mentionsThis polyclonal targets beta III Tubulin
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Chicken IgG (H+L)
View all literature mentionsThis polyclonal targets Rabbit IgG
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Goat IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets IgY (IgG) (H+L)
View all literature mentionsThis monoclonal targets CD133 (Prominin-1)
View all literature mentionsThis monoclonal targets CD73
View all literature mentionsThis polyclonal targets Rcvrn
View all literature mentionsThis monoclonal targets Dihydropyridine-sensitive Calcium Channel alpha 1 subunit
View all literature mentionsThis polyclonal targets Human RYK (C-term)
View all literature mentionsThis polyclonal targets RBPMS
View all literature mentionsThis polyclonal targets OPN1SW (N-20)
View all literature mentionsThis polyclonal targets Human Secretagogin
View all literature mentionsThis monoclonal targets Rhodopsin antibody [Rho 4D2]
View all literature mentionsThis monoclonal targets PSD-95
View all literature mentionsThis monoclonal targets PKC
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis polyclonal targets Cone Arrestin
View all literature mentionsThis polyclonal targets Calbindin D-28k
View all literature mentionsThis monoclonal targets Bassoon mAb (SAP7F407)
View all literature mentionsMus musculus with name B6.Cg-Tg(Hlxb9-GFP)1Tmj/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Pde6b+ Tg(Rho-icre)1Ck/Boc from IMSR.
View all literature mentionsThis polyclonal targets Rcvrn
View all literature mentionsThis polyclonal targets Cone Arrestin
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