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Dopamine controls essential brain functions through volume transmission. Different from fast synaptic transmission, where neurotransmitter release and receptor activation are tightly coupled by an active zone, dopamine transmission is widespread and may not necessitate these organized release sites. Here, we determine whether striatal dopamine secretion employs specialized machinery for release. Using super resolution microscopy, we identified co-clustering of the active zone scaffolding proteins bassoon, RIM and ELKS in ∼30% of dopamine varicosities. Conditional RIM knockout disrupted this scaffold and, unexpectedly, abolished dopamine release, while ELKS knockout had no effect. Optogenetic experiments revealed that dopamine release was fast and had a high release probability, indicating the presence of protein scaffolds for coupling Ca2+ influx to vesicle fusion. Hence, dopamine secretion is mediated by sparse, mechanistically specialized active zone-like release sites. This architecture supports spatially and temporally precise coding for dopamine and provides molecular machinery for regulation.
Pubmed ID: 29398114 RIS Download
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This monoclonal targets Mouse Rim Clone 26
View all literature mentionsThis polyclonal targets Tyrosine hydroxylase
View all literature mentionsThis polyclonal targets RIM1 central domains
View all literature mentionsThis unknown targets Bassoon mAb (SAP7F407)
View all literature mentionsThis polyclonal targets Synaptobrevin 2
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis polyclonal targets rat VMAT2 496-515
View all literature mentionsThis monoclonal targets Synaptophysin 1
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase
View all literature mentionsThis polyclonal targets VGLUT 1
View all literature mentionsThis polyclonal targets Dopamine Transporter (DAT)
View all literature mentionsThis polyclonal targets Munc 13-1
View all literature mentionsThis polyclonal targets Bassoon
View all literature mentionsMus musculus with name B6.SJL-Slc6a3tm1.1(cre)Bkmn/J from IMSR.
View all literature mentionsMus musculus with name B6;129S-Slc17a7tm1.1(cre)Hze/J from IMSR.
View all literature mentionsMus musculus with name STOCK Rims1tm3Sud/J from IMSR.
View all literature mentionsMus musculus with name STOCK Rims2tm1.1Sud/J from IMSR.
View all literature mentionsMus musculus with name STOCK Erc2tm1.2Sud/J from IMSR.
View all literature mentionsMus musculus with name STOCK Erc1tm2.1Sud/J from IMSR.
View all literature mentionsMus musculus with name B6;129S-Gt(ROSA)26Sortm34.1(CAG-Syp/tdTomato)Hze/J from IMSR.
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase
View all literature mentionsThis unknown targets Bassoon mAb (SAP7F407)
View all literature mentionsThis polyclonal targets Bassoon
View all literature mentionsThis monoclonal targets Mouse Rim Clone 26
View all literature mentionsThis polyclonal targets rat VMAT2 496-515
View all literature mentionsThis polyclonal targets RIM1 central domains
View all literature mentionsThis polyclonal targets Munc 13-1
View all literature mentionsMus musculus with name B6;129S-Slc17a7tm1.1(cre)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6;129S-Gt(ROSA)26Sortm34.1(CAG-Syp/tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name STOCK Erc2tm1.2Sud/J from IMSR.
View all literature mentionsMus musculus with name STOCK Erc1tm2.1Sud/J from IMSR.
View all literature mentionsMus musculus with name STOCK Rims2tm1.1Sud/J from IMSR.
View all literature mentionsMus musculus with name STOCK Rims1tm3Sud/J from IMSR.
View all literature mentionsMus musculus with name B6.SJL-Slc6a3tm1.1(cre)Bkmn/J from IMSR.
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis polyclonal targets Dopamine Transporter (DAT)
View all literature mentionsThis polyclonal targets VGLUT 1
View all literature mentionsThis monoclonal targets Synaptophysin 1
View all literature mentionsThis polyclonal targets Synaptobrevin 2
View all literature mentionsThis polyclonal targets Tyrosine hydroxylase
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