Plants initiate immunity by cell-surface pattern-recognition receptors (PRRs), which perceive non-self molecules. PRRs are predominantly receptor serine/threonine (Ser/Thr) kinases that are evolutionarily related to animal interleukin-1 receptor-associated kinase (IRAK)/Pelle-soluble kinases. However, how the activity of these receptor kinases is modulated remains poorly understood. We report that the Arabidopsis PRR chitin elicitor receptor kinase 1 (CERK1) is autophosphorylated in unstimulated cells at tyrosine428 (Tyr428), a modification that is required for CERK1 activation upon binding to the fungal cell wall component chitin. Upon chitin activation, CERK1 recruits the CERK1-interacting protein phosphatase 1 (CIPP1), a predicted Ser/Thr phosphatase, to dephosphorylate Tyr428 and dampen CERK1 signaling. CIPP1 subsequently dissociates from Tyr428-dephosphorylated CERK1, allowing CERK1 to regain Tyr428 autophosphorylation and return to a standby state. This work sheds light onto plant chitin signaling and shows that a receptor kinase and phosphatase can coordinately regulate signal transduction of a receptor kinase through a phosphorylation cycle.
Pubmed ID: 29396039 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This polyclonal targets Mouse IgG
View all literature mentionsThis polyclonal targets Rabbit IgG
View all literature mentionsThis unknown targets ANTI-FLAG(R) M2 Affinity Gel
View all literature mentionsThis polyclonal targets Erk1/2
View all literature mentionsThis monoclonal targets GST
View all literature mentionsThis monoclonal targets His Tag
View all literature mentionsThis monoclonal targets cMyc
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis unknown targets GFP
View all literature mentionsThis monoclonal targets Phosphotyrosine antibody produced in mouse
View all literature mentionsThis polyclonal targets Human Phosphoserine / threonine
View all literature mentions