The development of cell therapy for repairing damaged or diseased skeletal muscle has been hindered by the inability to significantly expand immature, transplantable myogenic stem cells (MuSCs) in culture. To overcome this limitation, a deeper understanding of the mechanisms regulating the transition between activated, proliferating MuSCs and differentiation-primed, poorly engrafting progenitors is needed. Here, we show that methyltransferase Setd7 facilitates such transition by regulating the nuclear accumulation of β-catenin in proliferating MuSCs. Genetic or pharmacological inhibition of Setd7 promotes in vitro expansion of MuSCs and increases the yield of primary myogenic cell cultures. Upon transplantation, both mouse and human MuSCs expanded with a Setd7 small-molecule inhibitor are better able to repopulate the satellite cell niche, and treated mouse MuSCs show enhanced therapeutic potential in preclinical models of muscular dystrophy. Thus, Setd7 inhibition may help bypass a key obstacle in the translation of cell therapy for muscle disease.
Pubmed ID: 29395054 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
A commercial graphing software company that offers scientific software for statistical analyses, curve fitting and data analysis. It offers four programs: Prism, InStat, StatMate and QuickCalcs.
View all literature mentionsSoftware tool to visualize and analyse high throughput mapped sequence data.
View all literature mentionsSoftware for aligning sequencing reads against large reference genome. Consists of three algorithms: BWA-backtrack, BWA-SW and BWA-MEM. First for sequence reads up to 100bp, and other two for longer sequences ranged from 70bp to 1Mbp.
View all literature mentionsThis monoclonal targets myosin, sarcomere (MHC)
View all literature mentionsThis monoclonal targets myosin (embryonic)
View all literature mentionsThis monoclonal targets SETD7 antibody [EPR5574]
View all literature mentionsThis monoclonal targets CD56
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis polyclonal secondary targets IgY (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Histone H3 (D1H2) XP Rabbit mAb
View all literature mentionsThis monoclonal targets H3K4me3
View all literature mentionsThis monoclonal targets Histone H3, dimethyl (Lys4)
View all literature mentionsThis polyclonal targets H3K4me1
View all literature mentionsThis polyclonal targets RCJMB04_4h19
View all literature mentionsThis polyclonal targets alpha Tubulin - Loading Control
View all literature mentionsThis polyclonal targets Laminin
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets c-Myc
View all literature mentionsThis unknown targets BCL9
View all literature mentionsThis monoclonal targets Raised against amino acids 680-781 mapping at the C-terminus of b-Cat o human origin
View all literature mentionsThis monoclonal targets Catenin, beta
View all literature mentionsThis polyclonal targets MyoD (C-20)
View all literature mentionsThis monoclonal targets Pax7
View all literature mentionsThis monoclonal targets CD29
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD34
View all literature mentionsThis monoclonal targets CD31
View all literature mentionsThis monoclonal targets Ly-6A/E (Sca-1)
View all literature mentionsThis monoclonal targets CD31 (PECAM-1)
View all literature mentionsThis unknown targets SET7 / SET9
View all literature mentionsThis monoclonal targets SETD7
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsBioconductor software package for Empirical analysis of Digital Gene Expression data in R. Used for differential expression analysis of RNA-seq and digital gene expression data with biological replication.
View all literature mentionsMus musculus with name FVB-Tg(CAG-luc.-GFP)L2G85Chco/J from IMSR.
View all literature mentionsMus musculus with name C57BL/6-Tg(CAG-EGFP)1Osb/J from IMSR.
View all literature mentionsMus musculus with name B6Ros.Cg-Dmdmdx-4Cv/J from IMSR.
View all literature mentions
Mus musculus with name NOD.Cg-Prkdcscid Il2rg
Mus musculus with name C57BL/10ScSn-Dmdmdx/J from IMSR.
View all literature mentionsMus musculus with name B6;129-Pax7tm2.1(cre/ERT2)Fan/J from IMSR.
View all literature mentionsThis monoclonal targets myosin (embryonic)
View all literature mentionsThis monoclonal targets myosin, sarcomere (MHC)
View all literature mentions