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Damaged mitochondria are selectively eliminated by mitophagy. Parkin and PINK1, gene products mutated in familial Parkinson's disease, play essential roles in mitophagy through ubiquitination of mitochondria. Cargo ubiquitination by E3 ubiquitin ligase Parkin is important to trigger selective autophagy. Although autophagy receptors recruit LC3-labeled autophagic membranes onto damaged mitochondria, how other essential autophagy units such as ATG9A-integrated vesicles are recruited remains unclear. Here, using mammalian cultured cells, we demonstrate that RABGEF1, the upstream factor of the endosomal Rab GTPase cascade, is recruited to damaged mitochondria via ubiquitin binding downstream of Parkin. RABGEF1 directs the downstream Rab proteins, RAB5 and RAB7A, to damaged mitochondria, whose associations are further regulated by mitochondrial Rab-GAPs. Furthermore, depletion of RAB7A inhibited ATG9A vesicle assembly and subsequent encapsulation of the mitochondria by autophagic membranes. These results strongly suggest that endosomal Rab cycles on damaged mitochondria are a crucial regulator of mitophagy through assembling ATG9A vesicles.
Pubmed ID: 29360040 RIS Download
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A commercial antibody supplier and provider of various services.
View all literature mentionsNon-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
View all literature mentionsCommercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.
View all literature mentionsA commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.
View all literature mentionsA contract research organization providing drug development and animal testing services. Under the name Covance Research Products Inc., based in Denver, Pennsylvania, the company also deals in the import, breeding and sale of laboratory animals. It breeds dogs, rabbits, guinea pigs, non-human primates, and pigs, and runs the largest non-human primate laboratory in Germany. (Wikipedia)
View all literature mentionsSoftware application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.
View all literature mentionsThis unknown targets LC3B
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis polyclonal targets Rabex5
View all literature mentionsThis monoclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets Mfn2
View all literature mentionsThis monoclonal targets anti-HA-tag
View all literature mentionsThis monoclonal targets actin
View all literature mentionsThis monoclonal targets RAB7
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Rab7
View all literature mentionsThis monoclonal targets Human LAMP2
View all literature mentionsThis monoclonal targets DNA clone AC-30-10
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6 ]
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis monoclonal targets Cytochrome C oxidase subunit II antibody [12C4F12 ]
View all literature mentionsThis polyclonal targets Tom20 (FL-145)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets Ubiquitin
View all literature mentionsThis monoclonal targets GM130
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis monoclonal targets Human TOMM20
View all literature mentionsThis monoclonal targets Rab5
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsCell line HCT 116 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentions3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsThis polyclonal targets Tom20 (FL-145)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HCT 116 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsThis monoclonal targets Mfn2
View all literature mentionsThis unknown targets LC3B
View all literature mentionsThis monoclonal targets Cytochrome C oxidase subunit II antibody [12C4F12 ]
View all literature mentionsThis monoclonal targets actin
View all literature mentionsThis monoclonal targets RAB7
View all literature mentionsCell line HCT 116 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets Ubiquitin
View all literature mentionsThis polyclonal targets Rabex5
View all literature mentionsThis polyclonal targets Tom20 (FL-145)
View all literature mentionsThis monoclonal targets Cytochrome C oxidase subunit II antibody [12C4F12 ]
View all literature mentionsThis monoclonal targets Mfn2
View all literature mentionsThis unknown targets LC3B
View all literature mentionsThis monoclonal targets actin
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis monoclonal targets Human TOMM20
View all literature mentionsThis monoclonal targets GM130
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis polyclonal targets Rabex5
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis monoclonal targets RAB7
View all literature mentionsThis monoclonal targets Ubiquitin
View all literature mentionsThis monoclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis monoclonal targets Human TOMM20
View all literature mentionsThis monoclonal targets Rab7
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis monoclonal targets Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6 ]
View all literature mentionsThis monoclonal targets Human LAMP2
View all literature mentionsThis monoclonal targets anti-HA-tag
View all literature mentionsThis monoclonal targets anti-HA-tag
View all literature mentionsThis monoclonal targets Human LAMP2
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentions3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Rab5
View all literature mentionsThis monoclonal targets DNA clone AC-30-10
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentions3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Rab7
View all literature mentionsThis monoclonal targets Rab5
View all literature mentionsThis monoclonal targets DNA clone AC-30-10
View all literature mentionsThis monoclonal targets Pyruvate Dehydrogenase E1-alpha subunit antibody [8D10E6 ]
View all literature mentionsThis monoclonal targets GM130
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