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Publication

Molecular Anatomy of the Developing Human Retina.

Developmental cell | 2017

Clinical and genetic heterogeneity associated with retinal diseases makes stem-cell-based therapies an attractive strategy for personalized medicine. However, we have limited understanding of the timing of key events in the developing human retina, and in particular the factors critical for generating the unique architecture of the fovea and surrounding macula. Here we define three key epochs in the transcriptome dynamics of human retina from fetal day (D) 52 to 136. Coincident histological analyses confirmed the cellular basis of transcriptional changes and highlighted the dramatic acceleration of development in the fovea compared with peripheral retina. Human and mouse retinal transcriptomes show remarkable similarity in developmental stages, although morphogenesis was greatly expanded in humans. Integration of DNA accessibility data allowed us to reconstruct transcriptional networks controlling photoreceptor differentiation. Our studies provide insights into human retinal development and serve as a resource for molecular staging of human stem-cell-derived retinal organoids.

Pubmed ID: 29233477 RIS Download

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Associated grants

Agency: Intramural NIH HHS, United States
Id: ZIA EY000474-08
Agency: Intramural NIH HHS, United States
Id: ZIA EY000546-02
Agency: Intramural NIH HHS, United States
Id: ZIA EY000450-09
Agency: NEI NIH HHS, United States
Id: F32 EY025117
Agency: NEI NIH HHS, United States
Id: P30 EY001730
Agency: NICHD NIH HHS, United States
Id: R24 HD000836
Agency: NIGMS NIH HHS, United States
Id: P01 GM081619
Agency: Intramural NIH HHS, United States
Id: Z01 EY000450

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