The striatum is the major component of the basal ganglia and is well known to play a key role in the control of motor function via balanced output from the indirect (iSPNs) and direct pathway striatal projection neurons (dSPNs). Little is known, however, about the molecular genetic mechanisms that control the formation of the iSPNs versus dSPNs. We show here that the SoxE family member, Sox8, is co-expressed with the dSPN markers, Isl1 and Ebf1, in the developing striatum. Moreover, dSPNs, as marked by Isl1-cre fate map, express Sox8 in the embryonic striatum and Sox8-EGFP BAC transgenic mice specifically reveal the direct pathway axons during development. These EGFP+ axons are first observed to reach their midbrain target, the substantia nigra pars reticulata (SNr), at E14 in the mouse with a robust connection observed already at birth. The selective expression of EGFP in dSPNs of Sox8-EGFP BAC mice is maintained at postnatal timepoints. Sox8 is known to be expressed in oligodendrocyte precursor cells (OPCs) together with other SoxE factors and we show here that the EGFP signal co-localizes with the OPC markers throughout the brain. Finally, we show that Sox8-EGFP BAC mice can be used to interrogate the altered dSPN development in Isl1 conditional mutants including aberrant axonal projections detected already at embryonic timepoints. Thus, Sox8 represents an early and specific marker of embryonic dSPNs and the Sox8-EGFP BAC transgenic mice are an excellent tool to study the development of basal ganglia circuitry.
Pubmed ID: 28472858 RIS Download
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This polyclonal targets amino acids 2-60 of mous Sox8
View all literature mentionsThis polyclonal targets PDGFRA
View all literature mentionsThis polyclonal targets Oligodendrocute transcription factor 2
View all literature mentionsThis polyclonal targets Sox-9 (H-90)
View all literature mentionsThis unknown targets Raised against peptide mapping to N-terminus of sox-10 of human origin
View all literature mentionsThis monoclonal targets Substance P
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase (TH)
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis polyclonal targets TTF-1
View all literature mentionsThis polyclonal targets Human Islet-1
View all literature mentionsMus musculus with name STOCK Tg(Dlx1-cre)RB27Gsat/Mmucd from MMRRC.
View all literature mentionsMus musculus with name B6.Cg-Tg(Drd1a-tdTomato)6Calak/J from IMSR.
View all literature mentionsMus musculus with name STOCK Tg(Sox8-EGFP)LY179Gsat/Mmucd from MMRRC.
View all literature mentionsMus musculus with name STOCK Tg(Drd1-EGFP)X60Gsat/Mmmh from MMRRC.
View all literature mentionsThis unknown targets Raised against peptide mapping to N-terminus of sox-10 of human origin
View all literature mentionsThis polyclonal targets Sox-9 (H-90)
View all literature mentionsThis monoclonal targets Substance P
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase (TH)
View all literature mentionsThis polyclonal targets Green Fluorescent Protein (GFP)
View all literature mentionsThis polyclonal targets PDGFRA
View all literature mentionsThis polyclonal targets Oligodendrocute transcription factor 2
View all literature mentionsThis polyclonal targets amino acids 2-60 of mous Sox8
View all literature mentions