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Publication

Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage.

eLife | 2017

Cartilaginous structures are at the core of embryo growth and shaping before the bone forms. Here we report a novel principle of vertebrate cartilage growth that is based on introducing transversally-oriented clones into pre-existing cartilage. This mechanism of growth uncouples the lateral expansion of curved cartilaginous sheets from the control of cartilage thickness, a process which might be the evolutionary mechanism underlying adaptations of facial shape. In rod-shaped cartilage structures (Meckel, ribs and skeletal elements in developing limbs), the transverse integration of clonal columns determines the well-defined diameter and resulting rod-like morphology. We were able to alter cartilage shape by experimentally manipulating clonal geometries. Using in silico modeling, we discovered that anisotropic proliferation might explain cartilage bending and groove formation at the macro-scale.

Pubmed ID: 28414273 RIS Download

Research resources used in this publication

Additional research tools detected in this publication

Allen Developing Mouse Brain Atlas (RRID:SCR_002990)

Antibodies used in this publication

Associated grants

Agency: Biotechnology and Biological Sciences Research Council, United Kingdom
Id: BBS/E/J/00000152
Agency: NIBIB NIH HHS, United States
Id: R01 EB014877

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This is a list of tools and resources that we have found mentioned in this publication.

Allen Developing Mouse Brain Atlas (tool)

RRID:SCR_002990

Map of gene expression in developing mouse brain revealing gene expression patterns from embryonic through postnatal stages. Provides information about spatial and temporal regulation of gene expression with database. Feature include seven sagittal reference atlases created with a developmental ontology. These anatomic atlases may be viewed alongside in situ hybridization (ISH) data as well as by itself.

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VECTASHIELD Mounting Medium with DAPI (antibody)

RRID:AB_2336790

This unknown targets

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Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (antibody)

RRID:AB_2536183

This polyclonal secondary targets IgG (H+L)

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Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555 (antibody)

RRID:AB_162543

This polyclonal secondary targets IgG (H+L)

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Sheep Anti-Mouse Erbb3 / her3 Affinity purified Polyclonal antibody, Unconjugated (antibody)

RRID:AB_2099728

This polyclonal targets Mouse ErbB3 / HER3

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SOX5 antibody (antibody)

RRID:AB_10859923

This polyclonal targets SOX5 antibody

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Anti-SOX9 antibody produced in rabbit (antibody)

RRID:AB_1080067

This polyclonal targets SOX9 antibody produced in rabbit

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Goat Anti-GFP (Green Fluorescent Protein) Polyclonal Antibody, Fluorescein Conjugated (antibody)

RRID:AB_305635

This polyclonal targets GFP (FITC)

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MATLAB (software resource)

RRID:SCR_001622

Multi paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.

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GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

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