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The c-Jun N-terminal kinases (JNKs) are important mediators of cell viability and structural integrity in postmitotic neurons, which is required for maintaining synaptic connections and neural plasticity. In the present study, we chose differentiated PC12 cells as a well-characterised neuronal model system to selectively examine the regulation of basal JNK activity by extracellular signal-regulated kinase 1/2 (ERK1/2) and Akt. We detected a complex interaction between the kinases to prevent cell death and neurite loss. Especially the appropriate level of JNK activation determined cellular survival. Basal activity of ERK1/2 attenuated the potentiation of JNK phosphorylation and thereby the induction of apoptosis. Importantly, when JNK activity was too low, cell viability and the number of neurite-bearing cells also decreased, even though the activation of ERK1/2 was enhanced. In this case, the JNK-mediated survival signals via activating transcription factor-3 (ATF3) were inhibited. Furthermore, the phosphorylation of ERK1/2 induced by the JNK inhibitor SP600125 inhibited the basal activity of Akt, which normally supported cell viability. Thus, controlling JNK activity is crucial to promote survival and neurite stability of differentiated neuronal cells.
Pubmed ID: 28414095 RIS Download
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THIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 4th,2023. It was integrated with Antibody Registry. The JCN antibody database is a listing of all antibodies used in JCN papers from 2006 onward. The catalog numbers and vendor information is included for all antibodies listed, and with a new collaboration with NIF''''s AntibodyRegistry, a unique identifier is also listed for each antibody. The Journal of Comparative Neurology requires rigorous characterization for all antibodies that are used in JCN papers. The antibodies in the The Journal of Comparative Neurology antibody database have in nearly all cases been described and characterized adequately according to the provided guidelines. This information can be used to identify a particular target immunohistochemically or to design an experiment using the antibody information. If you are looking for an antibody to identify a particular target immunohistochemically, this list is a good place to begin your search. We suggest you then look up the paper in which the antibody was used, to make sure that it will meet your needs and to verify its characterization. (The characterization of antibodies in JCN papers often goes well beyond the material published by the manufacturer, so that examining this information before you order an antibody can be very useful.) While we do not guarantee that these antibodies will identify only the intended target (that is a function of the actual experiment and controls), this is the most carefully verified list of antibodies that we are aware of, and we wanted to share this resource with our readers and authors.
View all literature mentionsThis polyclonal targets MKK7 / SKK4, phospho (Thr275 / Ser277)
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis monoclonal targets Phospho-Akt (Ser473)
View all literature mentionsThis polyclonal targets p44/42 MAPK (Erk1/2)
View all literature mentionsThis polyclonal targets Cleaved Caspase-3 (Asp175)
View all literature mentionsThis polyclonal targets Raf1
View all literature mentionsThis polyclonal targets p-MEK-4 (Thr 261)
View all literature mentionsThis polyclonal targets p-MEK-1/2 (Ser 218/Ser 222)
View all literature mentionsThis polyclonal targets ACTIVE& x00AE; JNK, (pTPpY)
View all literature mentionsThis polyclonal targets Erk1/2
View all literature mentionsThis unknown targets c-Jun, phospho (Ser63) II
View all literature mentionsThis polyclonal targets Phospho-ATF-2 (Thr71)
View all literature mentionsThis monoclonal targets Neurofilament 200 antibody produced in mouse
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets Epitope mapping near the C-terminus of MKP-1 of mouse origin
View all literature mentionsThis monoclonal targets MEK-1/2 (9G3)
View all literature mentionsThis monoclonal targets MAP2A 2B
View all literature mentionsThis polyclonal targets SAPK/JNK
View all literature mentionsThis polyclonal targets caspase-3
View all literature mentionsThis polyclonal targets BCL2
View all literature mentionsThis polyclonal targets ATF-3 (C-19)
View all literature mentionsThis monoclonal targets slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis polyclonal targets MKK7 / SKK4, phospho (Thr275 / Ser277)
View all literature mentionsThis monoclonal targets Phospho-Akt (Ser473)
View all literature mentionsThis polyclonal targets p44/42 MAPK (Erk1/2)
View all literature mentionsThis polyclonal targets Cleaved Caspase-3 (Asp175)
View all literature mentionsThis polyclonal targets MKK7 / SKK4, phospho (Thr275 / Ser277)
View all literature mentionsThis polyclonal targets p-MEK-1/2 (Ser 218/Ser 222)
View all literature mentionsThis polyclonal targets Erk1/2
View all literature mentionsThis polyclonal targets Phospho-ATF-2 (Thr71)
View all literature mentionsThis monoclonal targets Neurofilament 200 antibody produced in mouse
View all literature mentionsThis monoclonal targets slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH
View all literature mentionsThis polyclonal targets SAPK/JNK
View all literature mentionsThis polyclonal targets caspase-3
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