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Melanin-concentrating hormone axons, but not orexin or tyrosine hydroxylase axons, innervate the claustrum in the rat: An immunohistochemical study.

The Journal of comparative neurology | 2017

The claustrum is a small, elongated nucleus close to the external capsule and deep in the insular cortex. In rodents, this nucleus is characterized by a dense cluster of parvalbumin labeling. The claustrum is connected with the cerebral cortex. It does not project to the brainstem, but brainstem structures can influence this nucleus. To identify some specific projections from the lateral hypothalamus and midbrain, we analyzed the distribution of projections labeled with antibodies against tyrosine hydroxylase (TH), melanin-concentrating hormone (MCH), and hypocretin (Hcrt) in the region of the claustrum. The claustrum contains a significant projection by MCH axons, whereas it is devoid of TH projections. Unlike TH and MCH axons, Hcrt axons are scattered throughout the region. This observation is discussed mainly with regard to the role of the claustrum in cognitive functions and that of MCH in REM sleep. J. Comp. Neurol. 525:1489-1498, 2017. © 2016 Wiley Periodicals, Inc.

Pubmed ID: 27580962 RIS Download

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This is a list of tools and resources that we have found mentioned in this publication.


Monoclonal anti Parvalbumin (antibody)

RRID:AB_10000343

This monoclonal targets parvalbumin

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MCH Melanin-concentrating hormone (antibody)

RRID:AB_2616562

This polyclonal targets Synthetic Salmon MCH; full 17 amino acid sequence: DTMRKMVGRVYRPCWEV

View all literature mentions

Orexin-A (antibody)

RRID:AB_653610

This polyclonal targets peptide mapping at the C-terminus of human Orexin-A

View all literature mentions

ZEISS ZEN Microscopy Software (software resource)

RRID:SCR_013672

User interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.

View all literature mentions

ZEISS ZEN Microscopy Software (software resource)

RRID:SCR_013672

User interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.

View all literature mentions

Orexin-A (antibody)

RRID:AB_653610

This polyclonal targets peptide mapping at the C-terminus of human Orexin-A

View all literature mentions

MCH Melanin-concentrating hormone (antibody)

RRID:AB_2616562

This polyclonal targets Synthetic Salmon MCH; full 17 amino acid sequence: DTMRKMVGRVYRPCWEV

View all literature mentions

Monoclonal anti Parvalbumin (antibody)

RRID:AB_10000343

This monoclonal targets parvalbumin

View all literature mentions