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A postnatal peak in microglial development in the mouse hippocampus is correlated with heightened sensitivity to seizure triggers.

Brain and behavior | 2015

Explosive synaptogenesis and synaptic pruning occur in the hippocampus during the first two weeks of postnatal life, coincident with a heightened susceptibility to seizures in rodents. To determine the temporal correlation between microglial development and age-dependent susceptibility and response to seizures, we quantified developmental changes in basal microglia levels and seizure-induced microglial activation in the hippocampus of Cx3Cr1(GFP /+) transgenic mice.

Pubmed ID: 26807334 RIS Download

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Associated grants

  • Agency: NINDS NIH HHS, United States
    Id: R01 NS073768
  • Agency: NINDS NIH HHS, United States
    Id: R01NS073768

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A collection of tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows. It provides features to help users integrate flow systems into new application areas, including index sorting for stem cell and single-cell applications, as well as automation protocols for high-throughput and robotic laboratories.

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ImageJ (software resource)

RRID:SCR_003070

Open source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.

View all literature mentions

PE anti-mouse CD3epsilon (antibody)

RRID:AB_312673

This monoclonal targets CD3epsilon

View all literature mentions

APC anti-mouse Ly-6C (antibody)

RRID:AB_1732076

This monoclonal targets Ly-6C

View all literature mentions

Iba1 antibody (antibody)

RRID:AB_2224402

This polyclonal targets AIF1

View all literature mentions

BD FACSDiva Software (software resource)

RRID:SCR_001456

A collection of tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows. It provides features to help users integrate flow systems into new application areas, including index sorting for stem cell and single-cell applications, as well as automation protocols for high-throughput and robotic laboratories.

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GraphPad Prism (software resource)

RRID:SCR_002798

Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.

View all literature mentions

FlowJo (software resource)

RRID:SCR_008520

Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.

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