URL: http://www.addgene.org/98293
Proper Citation: RRID:Addgene_98293
Insert Name: P2a-blast
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30894629
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #52961); Backbone Size:14676; Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: Double stranded oligonucleotides encoding sgRNA sequences can be cloned between the BsmBI restriction sites as for lentiCRISPRv2. The second MluI site in the original lentiCRISPRv2 plasmid was destroyed by Klenow end-filling and religation after MluI digestion. For target guide (sgRNA) sequence cloning instructions, please see https://www.addgene.org/52961 for the Zhang's lab lentiCRISPR v2 guide. We recommend screening multiple colonies to ensure the plasmid has been isolated correctly.
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Source: Addgene