URL: http://www.addgene.org/91706
Proper Citation: RRID:Addgene_91706
Insert Name: pylS
Organism: Methanosarcina barkeri
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24222614
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid for encoding the Methanosarcina barkeri pyrrolysyl-tRNA synthetase (MbPylRS)/tRNA pair in mammalian cells which can incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into a in-frame amber codon site on a protein of interest.The aminoacyl-tRNA synthetase is engineered to recognize the UAA but not the natural native amino acids, and charge its cognate tRNA with the corresponding UAA. Then, the charged tRNA will insert the UAA at an in-frame amber codon position on a target gene.
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Source: Addgene