URL: http://www.addgene.org/70709
Proper Citation: RRID:Addgene_70709
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26524662
Vector Backbone Description: Backbone Marker:Joachim Messing (Addgene plasmid # 50005); Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Use BsmBI sites to insert sgRNA spacer sequence. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA
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Source: Addgene